Uygun Murat, Yavuz Osman Y, Uraş İsmail, Duman İbrahim G, Haltaş Hacer, Kömürcü Mahmut
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Clin Invest Med. 2016 Dec 1;39(6):27526.
Ankaferd Blood Stopper (ABS), which is a standardized mixture of herbal extracts obtained from five plants, has been proven as an efficient hemostatic agent and is still used in emergency situations. It is not known exactly if decreased bleeding has positive or negative effects on muscle healing and fibrosis, so the purpose of this study was to test the effect of ABS on muscle healing and morphology.
A total of 66 outbred Wistar rats were divided into three control and three experimental subgroups. In the experimental groups, ABS was sprayed on the cut surface of the soleus. In the control groups, a saline solution was sprayed on the cut surface of the soleus. Subgroups were euthanized after 2 weeks, 3 weeks and 4 weeks, respectively. In each subgroup, eight rats were used for the biomechanical study to determine muscle healing and three rats were used for the histopathological investigation.
Although muscle strength in the control groups was lower than that of the experimental groups in early weeks, no differences were found between the control and the experimental groups at 4 weeks.
ABS has no negative effect on muscle healing. We also observed that ABS accelerated muscle healing compared to the control group. ABS could be used in hemostasis of open fractures and elective orthopedic surgeries.
安卡非德止血剂（ABS）是一种从五种植物中提取的标准化草药提取物混合物，已被证明是一种有效的止血剂，目前仍用于紧急情况。目前尚不清楚出血减少对肌肉愈合和纤维化是有正面还是负面影响，因此本研究的目的是测试ABS对肌肉愈合和形态的影响。
总共66只远交系Wistar大鼠被分为三个对照组和三个实验组。在实验组中，将ABS喷洒在比目鱼肌的切割面上。在对照组中，将盐溶液喷洒在比目鱼肌的切割面上。分别在2周、3周和4周后对各亚组实施安乐死。在每个亚组中，八只大鼠用于生物力学研究以确定肌肉愈合情况，三只大鼠用于组织病理学研究。
虽然在早期几周对照组的肌肉力量低于实验组，但在4周时对照组和实验组之间未发现差异。
ABS对肌肉愈合没有负面影响。我们还观察到，与对照组相比，ABS加速了肌肉愈合。ABS可用于开放性骨折的止血和择期骨科手术。
