Gum Sang Il, Kim Yeoun-Hee, Jung Jae-Chang, Kim Im Gyu, Lee Jun Seok, Lee Kyoo Won, Park Young Jeung
Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea.
Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea; Korean Medicine (KM)-Application Centre, Korea Institute of Oriental Medicine (KIOM), 70, Chemdan-ro, Dong-gu, Daegu, 701-300, Republic of Korea.
Biochem Biophys Res Commun. 2017 Jan 22;482(4):1148-1153. doi: 10.1016/j.bbrc.2016.12.002. Epub 2016 Dec 2.
Cyclosporine A (CsA), an immunomodulatory drug, and is increasingly used to treat moderate dry eye syndrome and ocular surface inflammation. However, any inhibitory effect on differentiation of fibroblasts to myofibroblasts remains unclear. Here, we show that the inhibitory effect of CsA on transforming growth factor-beta2 (TGF-β2)-induced myofibroblasts in primary cultured human pterygium fibroblasts. CsA significantly decreased mRNA and protein expression of myofibroblast-related markers including α-SMA, laminin, and fibronectin. These findings were supported by the results from immunofluorescence staining. Taken together, these results indicate the therapeutic potential of CsA against pterygium progression. Further studies are necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced downregulation of myofibroblast markers in pterygium fibroblasts.
环孢素A（CsA）是一种免疫调节药物，越来越多地用于治疗中度干眼症和眼表炎症。然而，其对成纤维细胞向肌成纤维细胞分化的任何抑制作用仍不清楚。在此，我们展示了CsA对原代培养的人翼状胬肉成纤维细胞中转化生长因子-β2（TGF-β2）诱导的肌成纤维细胞的抑制作用。CsA显著降低了包括α-平滑肌肌动蛋白（α-SMA）、层粘连蛋白和纤连蛋白在内的肌成纤维细胞相关标志物的mRNA和蛋白质表达。免疫荧光染色结果支持了这些发现。综上所述，这些结果表明CsA对翼状胬肉进展具有治疗潜力。有必要进一步研究以阐明负责CsA诱导翼状胬肉成纤维细胞中肌成纤维细胞标志物下调的精确细胞内信号机制。
