Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu, China.
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 QiXiu Road, Nantong, 226001, Jiangsu, China.
Clin Exp Med. 2017 Nov;17(4):519-529. doi: 10.1007/s10238-016-0444-z. Epub 2016 Dec 5.
Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) has been known as an effector for the small GTPase Rho and plays an important role in tumor progression and metastasis. However, the effect of ROCK2 in gastric cancer (GC) has not been identified. This study showed that ROCK2 expression significantly increased in clinical GC tissues compared with adjacent non-cancer tissues. Immunohistochemistrical analysis showed that high expression of ROCK2 was correlated with tumor grade, tumor-node-metastasis stage, infiltration depth, lymph node invasion and Ki-67, and predicted poor prognosis in 135 gastric cancer specimens. In addition, we found that upregulated ROCK2 promoted proliferation, metastasis and invasion of GC cells, while ROCK2 knockdown led to the opposite results in vitro by Cell Counting Kit-8 (CCK-8) assay, colony formation assays, flow cytometric analysis and trans-well assays. Our findings supported that ROCK2 was a significant protein in the progress of GC and would provide a novel promising therapeutic strategy against human GC.
Rho 相关卷曲螺旋蛋白激酶 2（ROCK2）作为小 GTP 酶 Rho 的效应物而被熟知，在肿瘤进展和转移中发挥重要作用。然而，ROCK2 在胃癌（GC）中的作用尚未确定。本研究表明，与邻近非癌组织相比，ROCK2 在临床 GC 组织中的表达显著增加。免疫组织化学分析表明，ROCK2 的高表达与肿瘤分级、肿瘤-淋巴结-转移分期、浸润深度、淋巴结侵犯和 Ki-67 相关，并在 135 例胃癌标本中预测预后不良。此外，我们发现上调的 ROCK2 促进 GC 细胞的增殖、转移和侵袭，而 ROCK2 敲低在体外通过细胞计数试剂盒（CCK-8）检测、集落形成实验、流式细胞术分析和 Trans-well 实验得到相反的结果。我们的研究结果表明 ROCK2 是 GC 进展中的重要蛋白，为针对人类 GC 的新型有前途的治疗策略提供了依据。
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