Gao Huabin, Han Zhaoli, Bai Ruojing, Huang Shan, Ge Xintong, Chen Fanglian, Lei Ping
Department of Neurosurgery, Tianjin Neurological Institute General Hospital, Tianjin Medical University, Tianjin 300052, China.
Tianjin Institute of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300052, China.
Brain Res. 2017 Feb 15;1657:1-8. doi: 10.1016/j.brainres.2016.11.028. Epub 2016 Dec 5.
Traumatic brain injury (TBI) is a major public health problem with long-term neurobehavioral sequela. The evidences have revealed that TBI is a risk factor for later development of neurodegenerative disease and both the single and repetitive brain injury can lead to the neurodegeneration. But whether the effects of accumulation play an important role in the neurodegenerative disease is still unknown. We utilized the Sprague Dawley (SD) rats to develop the animal models of repetitive mild TBI and single mild TBI in order to detect the neurobehavioral changes. The results of neurobehavioral test revealed that the repetitive mild TBI led to more severe behavioral injuries than the single TBI. There were more activated microglia cells and astrocytes in the repetitive mild TBI group than the single TBI group. In consistent with this, the levels of TNF-α and IL-6 were higher and the expression of IL-10 was lower in the repetitive mild TBI group compared with the single TBI group. The expression of amyloid precursor protein (APP) increased in the repetitive TBI group detected by ELISA and western blot. But the levels of total tau (Tau-5) and P-tau (ser202) seem no different between the two groups in most time point. In conclusion, repetitive mild TBI could lead to more severe neurobehavioral impairments and the effects of accumulation may be associated with the increased inflammation in the brain.
创伤性脑损伤（TBI）是一个伴有长期神经行为后遗症的重大公共卫生问题。证据表明，TBI是神经退行性疾病后期发展的一个危险因素，单次和重复性脑损伤均可导致神经退行性变。但累积效应在神经退行性疾病中是否起重要作用仍不清楚。我们利用Sprague Dawley（SD）大鼠建立重复性轻度TBI和单次轻度TBI动物模型，以检测神经行为变化。神经行为测试结果显示，重复性轻度TBI比单次TBI导致更严重的行为损伤。重复性轻度TBI组比单次TBI组有更多活化的小胶质细胞和星形胶质细胞。与此一致的是，重复性轻度TBI组的TNF-α和IL-6水平较高，而IL-10的表达较低。通过ELISA和western blot检测发现，重复性TBI组淀粉样前体蛋白（APP）的表达增加。但在大多数时间点，两组之间总tau（Tau-5）和磷酸化tau（ser202）水平似乎没有差异。总之，重复性轻度TBI可导致更严重的神经行为损害，累积效应可能与大脑炎症增加有关。
