Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Sci Rep. 2016 Dec 7;6:38114. doi: 10.1038/srep38114.
A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL to 10 ng mL, and the limit of detection (LOD) was 0.02 ng mL, which was 10-20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.
本报告介绍了一种小分子的新型检测方法，即竞争生物条码放大免疫分析。通过我们制备的金纳米粒子 (AuNPs) 探针和磁性纳米粒子 (MNPs) 探针，仅使用一种单克隆抗体即可检测小分子。竞争生物条码放大免疫分析克服了生物条码分析不能用于小分子检测的障碍，因为由于小分子的结构，两个抗体无法结合到一个小分子上。选择三唑磷作为竞争生物条码放大免疫分析的靶标小分子化合物。检测的线性范围为 0.04ng/mL 至 10ng/mL，检测限（LOD）为 0.02ng/mL，比 ELISA（酶联免疫吸附测定）低 10-20 倍。通过检测实际样品中的三唑磷，评估了所提出的免疫分析的实际应用。回收率在 72.5%至 110.5%之间，相对标准偏差（RSD）小于 20%。这些结果通过 GC-MS 进行了验证，表明这种方便、灵敏的方法在实际样品中小分子具有很大的应用潜力。
