Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, China.
Mikrochim Acta. 2022 Feb 21;189(3):114. doi: 10.1007/s00604-022-05217-5.
A rapid detection method is introduced for residual trace levels of triazophos in water and agricultural products using an immunoassay based on catalytic hairpin self-assembly (CHA). The gold nanoparticle (AuNPs) surface was modified with triazophos antibody and sulfhydryl bio-barcode, and an immune competition reaction system was established between triazophos and its ovalbumin-hapten (OVA-hapten). The bio-barcode served as a catalyst to continuously induce the CHA reaction to achieve the dual signal amplification. The method does not rely on the participation of enzymes, and the addition of fluorescent materials in the last step avoids interfering factors, such as a fluorescence burst. The emitted fluorescence was detected at 489/521 nm excitation/emission wavelengths. The detection range of the developed method was 0.01-50 ng/mL for triazophos, and the limit of detection (LOD) was 0.0048 ng/mL. The developed method correlates well with the results obtained by LC-MS/MS, with satisfactory recovery and sensitivity. In sum, the designed method is reliable and provides a new approach to detect pesticide residues rapidly and quantitatively.
本文介绍了一种基于催化发夹自组装（CHA）的免疫测定法，用于快速检测水中和农产品中残留的三唑磷痕量。金纳米粒子（AuNPs）表面修饰有三唑磷抗体和巯基生物条码，并在三唑磷与其卵清蛋白半抗原（OVA-hapten）之间建立了免疫竞争反应体系。生物条码作为催化剂，不断诱导 CHA 反应，实现双重信号放大。该方法不依赖酶的参与，且最后一步添加荧光物质避免了荧光爆发等干扰因素。在 489/521nm 的激发/发射波长下检测到发射荧光。该方法对三唑磷的检测范围为 0.01-50ng/mL，检测限（LOD）为 0.0048ng/mL。所开发的方法与 LC-MS/MS 获得的结果相关性良好，具有令人满意的回收率和灵敏度。总之，该设计方法可靠，为快速定量检测农药残留提供了一种新方法。
