Carattino Aquiles, Keizer Veer I P, Schaaf Marcel J M, Orrit Michel
Leiden Institute of Physics, Leiden, the Netherlands.
Institute of Biology and Molecular Cell Biology, University of Leiden, Leiden, the Netherlands.
Biophys J. 2016 Dec 6;111(11):2492-2499. doi: 10.1016/j.bpj.2016.10.035.
Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by detecting their anti-Stokes emission under resonant excitation. We show that even in the membrane of a cell containing the fluorescent dye Atto 647N, the signal/background of the anti-Stokes emission can be >10, while it is impossible to image the particles with the Stokes emission. The main advantage of this technique is that it does not require any major change in existing fluorescence imaging setups, only the addition of an appropriate short-pass filter in the detection path.
金属纳米颗粒开启了对生物样品进行无时间限制成像、追踪和操控的可能性。然而，它们较低的光致发光量子产率使得在高背景条件下难以检测到它们。在本研究中，我们表明通过在共振激发下检测金纳米棒的反斯托克斯发射来对其成像成为可能。我们表明，即使在含有荧光染料Atto 647N的细胞膜中，反斯托克斯发射的信号/背景也可以大于10，而用斯托克斯发射对这些颗粒进行成像则是不可能的。这项技术的主要优点是它不需要对现有的荧光成像装置进行任何重大改变，只需在检测路径中添加一个合适的短通滤波器即可。
