Ataee Ramezan Ali, Mehrabi-Tavana Ali, Hosseini Seyed Mohammad Javad, Kaviani Farshad
Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Health Management Research Center and Department of Medical Microbiology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2016 Jun;8(3):203-209.
is transmitted from person-to-person. Thus, close contact with a healthy carrier can facilitate the spread of the bacteria and lead to life-threatening meningococcal disease. The aim of this study was to identify oropharyngeal carriers of in volunteers preparing for military service before vaccination.
In a cross-sectional study, 226 volunteers entering military service were referred to the Shemiranat Health Center for meningococcal vaccination and assayed. Before vaccination, the participants underwent sampling of the throat using separate swabs. Thayer-Martin Agar medium and microbiological standard methods were used for culture and isolation of the organisms. The bacterial isolates were subjected to DNA extraction and polymerase chain reaction. The obtained data were descriptively analyzed.
Out of the 226 (100%) young volunteers, only 18 (8%) yielded Gram-negative diplococci. The results showed the presence of (carriage rate: 8%) in their oropharyngeal regions. The isolated serogroups were C, A, Y, W-135, and X with frequencies of 50, 22.2, 16.6, 5.5, and 5.5, respectively.
This study showed that the carriage rate in young volunteers for military service is around 8% before vaccination. Although the rates for serogroups A and C were dominant, the existence of serogroups Y and W indicate the necessary revision of the A/C vaccine. More research is needed to determine serogroup diversity and decrease the risk of meningococcal disease in individual groups.
[疾病名称]通过人与人之间传播。因此，与健康携带者的密切接触会促进细菌传播，并导致危及生命的脑膜炎球菌病。本研究的目的是在准备服兵役的志愿者接种疫苗前，识别其口咽部的[细菌名称]携带者。
在一项横断面研究中，226名即将服兵役的志愿者被转至舍米拉纳特健康中心进行脑膜炎球菌疫苗接种并接受检测。在接种疫苗前，参与者使用单独的拭子进行咽部采样。采用蒂尔-马丁琼脂培养基和微生物学标准方法进行微生物培养和分离。对细菌分离株进行DNA提取和聚合酶链反应。对获得的数据进行描述性分析。
在226名（100%）年轻志愿者中，只有18名（8%）培养出革兰氏阴性双球菌。结果显示他们的口咽部存在[细菌名称]（携带率：8%）。分离出的血清群为C、A、Y、W-135和X，频率分别为50%、22.2%、16.6%、5.5%和5.5%。
本研究表明，准备服兵役的年轻志愿者在接种疫苗前的携带率约为8%。虽然A群和C群血清型的携带率占主导，但Y群和W群血清型的存在表明有必要对A/C疫苗进行修订。需要更多研究来确定血清群的多样性，并降低个体群体中脑膜炎球菌病的风险。
