De Luise M, Harker M
University of Melbourne, Department of Medicine, Repatriation General Hospital, Heidelberg, Australia.
Horm Metab Res. 1989 Aug;21(8):410-5. doi: 10.1055/s-2007-1009250.
To investigate the potential role of skeletal muscle metabolism in the determination of whole body energy balance, the rate of oxygen consumption was measured in soleus muscles isolated from mice of differing age, sex, strain (thin and obese), thyroid and nutritional status. As expected, T3-induced hyperthyroidism resulted in an increase in the intrinsic rate of energy utilization by the muscles; qualitatively similar changes were noted in both younger animals and mice induced to increase their energy intake by sucrose-overfeeding. In contrast, soleus muscle oxygen consumption was significantly reduced in genetically obese NZO mice. Parallel changes in the activity of the Na-K pump were observed in all groups of animals, with a good correlation (r greater than 0.6, P less than 0.01) noted between these two independently measured aspects of muscle metabolism. The results suggest that the intrinsic metabolic rate of the skeletal muscle mass plays an important role in the control of overall energy balance, with changes in this rate being potentially partly responsible for the altered energetic efficiency of genetic obesity and adaptive thermogenesis models. In addition, measurement of the activity of the Na-K-pump may provide a convenient marker for such observed changes in energy utilization.
为了研究骨骼肌代谢在全身能量平衡调节中的潜在作用，对来自不同年龄、性别、品系（瘦型和肥胖型）、甲状腺及营养状态的小鼠分离出的比目鱼肌的耗氧率进行了测量。正如预期的那样，T3诱导的甲状腺功能亢进导致肌肉内在能量利用率增加；在年幼动物以及通过过量喂食蔗糖诱导增加能量摄入的小鼠中也观察到了定性相似的变化。相比之下，遗传性肥胖的NZO小鼠的比目鱼肌耗氧量显著降低。在所有动物组中均观察到钠钾泵活性的平行变化，在这两个独立测量的肌肉代谢方面之间存在良好的相关性（r大于0.6，P小于0.01）。结果表明，骨骼肌的内在代谢率在总体能量平衡的控制中起重要作用，该速率的变化可能部分导致了遗传性肥胖和适应性产热模型中能量效率的改变。此外，测量钠钾泵的活性可能为所观察到的能量利用变化提供一个方便的标志物。
