Ahlman H, Ahlund L, Nilsson O, Dahlström A, Bjartell A, Ekman R
Department of Surgery, University of Göteborg, Sweden.
Int J Cancer. 1989 Oct 15;44(4):641-7. doi: 10.1002/ijc.2910440414.
By immunocytochemistry, delta sleep-inducing peptide (DSIP) was demonstrated to coexist with serotonin (5-HT) in a majority of midgut carcinoid tumour cells studied in biopsies and long-term cell cultures. Tumour cell colonies were characterized ultrastructurally and by confocal laser microscopy. The cultures produced several DSIP-like peptides chromatographically separated from culture media. DSIP has not yet proved to be a useful tumour marker clinically. Provocation with pentagastrin in patients with midgut carcinoid syndrome resulted in increased peripheral levels of 5HT, but not of DSIP.
通过免疫细胞化学方法，在活检及长期细胞培养中所研究的大多数中肠类癌肿瘤细胞中，发现诱导δ波睡眠肽（DSIP）与血清素（5-羟色胺，5-HT）共存。通过超微结构和共聚焦激光显微镜对肿瘤细胞集落进行了表征。这些培养物产生了几种经色谱法从培养基中分离出来的类似DSIP的肽。DSIP在临床上尚未被证明是一种有用的肿瘤标志物。对中肠类癌综合征患者用五肽胃泌素进行激发试验，结果外周血5-羟色胺水平升高，但DSIP水平未升高。
