Vincent-Hubert Françoise, Uher Emmanuelle, Di Giorgio Carole, Michel Cécile, De Meo Michel, Gourlay-France Catherine
IRSTEA, UR Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030, 92761, Antony cedex, France.
Present address: IFREMER, Laboratoire de Microbiologie - LNR, rue de l'île d'Yeu, BP 21105, F 44311, Nantes, Cedex 03, France.
Ecotoxicology. 2017 Mar;26(2):165-172. doi: 10.1007/s10646-016-1751-6. Epub 2016 Dec 8.
The genotoxicity of river water dissolved contaminants is usually estimated after grab sampling of river water. Water contamination can now be obtained with passive samplers that allow a time-integrated sampling of contaminants. Since it was verified that low density polyethylene membranes (LDPE) accumulate labile hydrophobic compounds, their use was proposed as a passive sampler. This study was designed to test the applicability of passive sampling for combined chemical and genotoxicity measurements. The LDPE extracts were tested with the umu test (TA1535/pSK1002 ± S9) and the Ames assay (TA98, TA100 and YG1041 ± S9). We describe here this new protocol and its application in two field studies on four sites of the Seine River. Field LDPE extracts were negative with the YG1041 and TA100 and weakly positive with the TA98 + S9 and Umu test. Concentrations of labile mutagenic PAHs were higher upstream of Paris than downstream of Paris. Improvement of the method is needed to determine the genotoxicity of low concentrations of labile dissolved organic contaminants.
通常在采集河水的瞬时水样后，对河水中溶解污染物的遗传毒性进行评估。现在可以使用被动采样器获取水污染情况，这种采样器能够对污染物进行时间积分采样。由于已证实低密度聚乙烯膜（LDPE）会积累不稳定的疏水化合物，因此建议将其用作被动采样器。本研究旨在测试被动采样在化学和遗传毒性联合测量中的适用性。用umu试验（TA1535/pSK1002 ± S9）和艾姆斯试验（TA98、TA100和YG1041 ± S9）对LDPE提取物进行测试。我们在此描述这一新方案及其在塞纳河四个地点的两项实地研究中的应用。实地LDPE提取物在YG1041和TA100测试中呈阴性，在TA98 + S9和umu试验中呈弱阳性。不稳定诱变多环芳烃的浓度在巴黎上游高于巴黎下游。需要改进该方法以确定低浓度不稳定溶解有机污染物的遗传毒性。
