Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
U.S. Environmental Protection Agency, National Risk Management Research Laboratory, Land Remediation and Pollution Control Division, Cincinnati, Ohio 45224-1701, United States.
Environ Sci Technol. 2016 Dec 6;50(23):13086-13094. doi: 10.1021/acs.est.6b04059. Epub 2016 Nov 22.
In this study, the in vitro bioaccessibility (IVBA) of lead (Pb) in phosphate-amended Pb-contaminated soil was assessed using a variety of IVBA assays with an overarching aim of determining whether changes in Pb IVBA were congruent to those observed for Pb relative bioavailability (RBA) determined using an in vivo mouse assay. Amending soil with phosphoric acid or rock phosphate resulted in changes in Pb speciation, however, varying Pb IVBA results were obtained depending on the methodology utilized. In addition, IVBA assays influenced Pb speciation as a consequence of interactions between dissolved Pb and unreacted phosphate arising from the amendment or from assay constituents. When the relationship between Pb RBA and IVBA was assessed, a comparison of treatment effect ratios (Pb RBA or IVBA in treated soil divided by Pb RBA or IVBA for untreated soil) provided the best in vivo-in vitro correlation particular for SBRC (r = 0.83) and IVG (r = 0.89) intestinal extraction. For these assays, the slope of the lines of best fit were close to 1 (1.12, 0.82; SBRC, IVG intestinal extraction respectively) with small y-intercepts (0.09, 0.08 respectively) indicating that the efficacy of phosphate amendments for reducing Pb RBA may be predicted using IVBA assays.
在这项研究中，使用多种体外生物可给性（IVBA）测定方法评估了磷酸盐修饰的 Pb 污染土壤中 Pb 的体外生物可给性（IVBA），其总体目标是确定 Pb IVBA 的变化是否与使用体内小鼠测定法确定的 Pb 相对生物可利用性（RBA）的变化一致。用磷酸或磷矿粉对土壤进行修饰会改变 Pb 的形态，但根据所用方法的不同，得到的 Pb IVBA 结果也不同。此外，由于来自修饰剂或测定成分的溶解 Pb 和未反应的磷酸盐之间的相互作用，IVBA 测定法会影响 Pb 的形态。当评估 Pb RBA 和 IVBA 之间的关系时，处理效果比（处理土壤中的 Pb RBA 或 IVBA 除以未处理土壤中的 Pb RBA 或 IVBA）的比较提供了体内-体外相关性的最佳比较，特别是对于 SBRC（r = 0.83）和 IVG（r = 0.89）肠提取。对于这些测定，最佳拟合线的斜率接近 1（1.12，0.82；SBRC 和 IVG 肠提取分别），y 轴截距较小（0.09，0.08 分别），这表明磷酸盐修饰剂降低 Pb RBA 的效果可以使用 IVBA 测定法来预测。
