Sertich P L
Hofmann Center of the Section of Reproductive Studies, University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, Kennett Square, 19348.
J Am Vet Med Assoc. 1989 Oct 1;195(7):940-4.
Pregnancy was established by transcervical transfer of embryos from performance mares into recipient mares. Estrus was synchronized between donor (n = 17) and recipient (n = 43) mares. After a greater than or equal to 25-mm follicle was detected, donor mares were bred artificially daily until ovulation. Day of ovulation was recorded. Uterine flushes (n = 111) were performed on donor mares 7 days after ovulation, and recovered embryos were transferred transcervically to recipient mares within 2 hours. Embryos were recovered from 40.5% of uterine flushes. Of transferred single embryos, 65.7% resulted in pregnancy, detectable by ultrasonographic examination 23 days after transfer. Only 35.3% of twin embryos resulted in pregnancy. Results over a 4-year period were as follows: uteri were flushed on 14, 44, 31 and 22 occasions, and 8, 21, 15, and 11 embryos were recovered (1 embryo was not transferred), with 6, 11, 4, and 6 resulting in 30-day pregnancy in years 1 to 4, respectively.
通过将供体母马的胚胎经宫颈移植到受体母马体内来建立妊娠。供体母马（n = 17）和受体母马（n = 43）的发情周期同步。在检测到卵泡直径大于或等于25毫米后，每天对供体母马进行人工授精，直至排卵，并记录排卵日期。排卵后7天对供体母马进行子宫冲洗（n = 111），回收的胚胎在2小时内经宫颈移植到受体母马体内。40.5%的子宫冲洗回收了胚胎。移植的单个胚胎中，65.7%成功妊娠，移植后23天通过超声检查可检测到。双胎胚胎只有35.3%成功妊娠。4年期间的结果如下：分别在14、44、31和22次进行子宫冲洗，回收的胚胎分别为8、21、15和11个（1个胚胎未移植），第1至4年分别有6、11、4和6个胚胎妊娠至30天。
