Squires E L, McKinnon A O, Carnevale E M, Morris R, Nett T M
Animal Reproduction Laboratory, Colorado State University, Fort Collins 80523.
J Reprod Fertil Suppl. 1987;35:399-403.
For embryos collected from mares 7 days after ovulation, embryo recovery for single-ovulating mares was 53% compared to 106% for double-ovulating mares. Pregnancy rates 50 days after surgical transfer were 68 and 129%, respectively. Concentrations of LH were similar during the periovulatory period for cycles which included single or double ovulations. Horse pituitary extract given for 5.5 days resulted in greater than or equal to 2 ovulations (mean 3.8) in 26 of 28 mares and 2.0 embryos were recovered per donor compared to 0.65 for controls. Non-surgical pregnancy rates for embryos collected from superovulated mares 6 and 8 days after ovulation (18 of 46; 39%) were identical to those obtained for untreated controls (12 of 31; 39%). The overall pregnancy rate for superovulated donors, which provided 2-4 embryos, was 120%. Since the viability of multiple embryos collected from spontaneous and induced multiple-ovulating mares was equal to that of embryos from single-ovulating mares, collection and transfer of embryos from these mares will increase the efficiency of horse embryo transfer.
对于排卵后7天从母马采集的胚胎,单排卵母马的胚胎回收率为53%,而双排卵母马为106%。手术移植后50天的妊娠率分别为68%和129%。在包括单排卵或双排卵的周期中,围排卵期LH浓度相似。给予马垂体提取物5.5天,28匹母马中有26匹出现大于或等于2次排卵(平均3.8次),每个供体回收2.0个胚胎,而对照组为0.65个。排卵后6天和8天从超排母马采集的胚胎的非手术妊娠率(46匹中的18匹;39%)与未处理对照组(31匹中的12匹;39%)相同。提供2 - 4个胚胎的超排供体的总体妊娠率为120%。由于从自然和诱导双排卵母马采集的多个胚胎的活力与单排卵母马的胚胎相同,从这些母马采集和移植胚胎将提高马胚胎移植的效率。