Liang Ruoyu, He Jiao, Shi Yajuan, Li Zhifen, Sarvajayakesavalu Suriyanarayanan, Baninla Yvette, Guo Feifan, Chen Juan, Xu Xiangbo, Lu Yonglong
State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
College of Resources and Environmental Sciences, China Agriculture University, Beijing, 100193, China.
Chemosphere. 2017 Feb;168:1613-1618. doi: 10.1016/j.chemosphere.2016.11.147. Epub 2016 Dec 7.
In recent years, Perfluorooctane sulfonate (PFOS) was widely detected in Yellow-Bohai Sea and other areas, causing a series of adverse effects in aquatic organisms. However, present studies of its chronic and acute toxicity on aquatic organisms were far more inadequate. Therefore, in the present study, Daphnia magna was used to investigate PFOS toxicity on their immobilization, heartbeat, reproductive and biochemical performance in acute, subchronic and chronic exposure. The results showed that the 48h-EC value for immobilization was 79.35 mg L and the toxicity was classified as intermediate. Heartbeat was significantly stimulated and reproductive parameters were significantly suppressed by PFOS, which can be used to reflect the toxicological effects on individuals. On the other hand, intrinsic rate of natural increase was more sensitive than reproductive parameters, which indicated negative responses on population dynamics of Daphnia magna. In addition, there were different degrees of inhibition on GST, CAT and ChE activity, which indicated three types of enzyme could become biomarkers to chronic PFOS exposure. Most of selected and evaluated endpoints have significant sensitivity to PFOS at the concentration of 8 mg L during subchronic and chronic exposure.
近年来，全氟辛烷磺酸（PFOS）在黄渤海等海域被广泛检测到，对水生生物造成了一系列不利影响。然而，目前关于其对水生生物的慢性和急性毒性的研究还远远不够充分。因此，在本研究中，利用大型溞来研究PFOS在急性、亚慢性和慢性暴露条件下对其活动抑制、心跳、繁殖和生化性能的毒性。结果表明，活动抑制的48h-EC值为79.35mg/L，毒性分类为中等。PFOS显著刺激了心跳并显著抑制了繁殖参数，这可用于反映对个体的毒理学效应。另一方面，内禀自然增长率比繁殖参数更敏感，这表明对大型溞的种群动态有负面反应。此外，对谷胱甘肽-S-转移酶（GST）、过氧化氢酶（CAT）和胆碱酯酶（ChE）活性有不同程度的抑制，这表明这三种酶可成为慢性PFOS暴露的生物标志物。在亚慢性和慢性暴露期间，大多数选定和评估的终点在8mg/L浓度下对PFOS具有显著敏感性。
