Virtanen Anita, Toimela Tarja, Tihtonen Kati, Sarkanen Jertta-Riina, Huttala Outi, Heinonen Tuula, Uotila Jukka
Department of Obstetrics and Gynaecology, Tampere University Central Hospital, Tampere, Finland.
FICAM, Finnish Centre for Alternative Methods, School of Medicine, University of Tampere, Finland.
Pregnancy Hypertens. 2016 Oct;6(4):367-373. doi: 10.1016/j.preghy.2016.08.239. Epub 2016 Aug 28.
To explore in vitro angiogenic properties of maternal and umbilical cord blood sera from women with symptomatic pre-eclampsia in comparison with sera from women with normotensive pregnancies.
Maternal and umbilical blood serum samples were collected from eleven primiparous women with pre-eclampsia and ten healthy gestational-age-matched primiparous controls. The samples were tested for tubule formation in two different types of in vitro angiogenesis tests. The first test (fibroblast-HUVEC) showed effects on angiogenesis and the second test (hASC-HUVEC), in addition to angiogenesis, also showed effects on vasculogenesis. The pro-angiogenic and inhibitory properties of the samples were microscopically quantified after immunostaining tubular structures, using markers for von Willebrand factor (vWf) and collagen IV.
Serum samples from pre-eclamptic women inhibited tubule formation in both models, while those from normal pregnancy didn't. Umbilical blood samples were inhibitory both after pre-eclampsia and normal pregnancy. In the fibroblast-HUVEC model the inhibition was stronger after preeclampsia pregnancy, and the difference between groups was statistically significant. In the pre-eclampsia group a correlation between the inhibitory effect of umbilical blood and birth weight adjusted to gestational age was found. No clear correlation between sera from pregnant women and corresponding umbilical sera was found.
The strong inhibitory effect of maternal serum samples on tubule formation reflects the anti-angiogenic state that is present in pre-eclampsia.
探讨有症状子痫前期孕妇的母血和脐血血清与血压正常孕妇血清相比的体外血管生成特性。
收集11例子痫前期初产妇和10例孕周匹配的健康初产对照孕妇的母血和脐血血清样本。在两种不同类型的体外血管生成试验中检测样本的小管形成情况。第一个试验（成纤维细胞-人脐静脉内皮细胞）显示对血管生成有影响，第二个试验（人脂肪干细胞-人脐静脉内皮细胞）除血管生成外，还显示对血管发生有影响。使用血管性血友病因子（vWf）和IV型胶原标记物对管状结构进行免疫染色后，在显微镜下对样本的促血管生成和抑制特性进行定量分析。
子痫前期孕妇的血清样本在两种模型中均抑制小管形成，而正常妊娠孕妇的血清样本则无此作用。子痫前期和正常妊娠后的脐血样本均具有抑制作用。在成纤维细胞-人脐静脉内皮细胞模型中，子痫前期妊娠后的抑制作用更强，组间差异有统计学意义。在子痫前期组中，发现脐血的抑制作用与根据孕周调整的出生体重之间存在相关性。未发现孕妇血清与相应脐血血清之间有明显相关性。
孕妇血清样本对小管形成的强烈抑制作用反映了子痫前期存在的抗血管生成状态。
