Nayudu P L, Lopata A, Jones G M, Gook D A, Bourne H M, Sheather S J, Brown T C, Johnston W I
Reproductive Biology Unit, Royal Women's Hospital, Victoria, Australia.
Hum Reprod. 1989 Jul;4(5):558-67. doi: 10.1093/oxfordjournals.humrep.a136944.
The aim of this study was to determine whether certain characteristics of the follicular biochemistry, previously shown by us to be associated with oocyte developmental capacity, also reflected differences in oocyte appearance, and to determine the range of oocyte characteristics induced by ovarian stimulation. A representative sample of 33 human oocytes and associated follicular fluids was obtained after a follicular growth period considered suitable for IVF. Individual follicular fluid protein and proteoglycan levels, and follicular volume were compared with the morphological characteristics of each oocyte. Oocytes which retained the germinal vesicle nuclear status after exposure to human chorionic gonadotrophin tended to occur in small follicles (less than or equal to 2 ml) and to be highly vacuolated and with characteristic predicted a low potential for cleavage. Among those oocytes which had progressed to MII nuclear maturity [in the medium (2.5-6.5 ml) and large (greater than or equal to 7 ml) volume follicles] the degree of oocyte vacuolation was negatively correlated with alpha 1-antitrypsin level, while the degree of organelle clumping was correlated with proteoglycan and immunoglobulin levels. Only five of the oocytes (15%) in this sample had follicular characteristics consistent with a normal potential for pregnancy. These MII oocytes occurred within the medium volume range, had low vacuolation levels, and a low degree of organelle clumping. In contrast, those oocytes with a low potential for cleavage based on their follicular biochemistry, showed high cytoplasmic vacuolation levels.
本研究的目的是确定卵泡生物化学的某些特征（我们之前已证明其与卵母细胞发育能力相关）是否也反映了卵母细胞外观的差异，并确定卵巢刺激所诱导的卵母细胞特征范围。在一个被认为适合体外受精的卵泡生长周期后，获取了33个人类卵母细胞及相关卵泡液的代表性样本。将各个卵泡液中的蛋白质和蛋白聚糖水平以及卵泡体积与每个卵母细胞的形态特征进行比较。在接触人绒毛膜促性腺激素后仍保留生发泡核状态的卵母细胞往往出现在小卵泡（小于或等于2毫升）中，且高度空泡化，其特征预示着低卵裂潜力。在那些已发育至第二次减数分裂中期核成熟的卵母细胞中（存在于中等体积卵泡（2.5 - 6.5毫升）和大体积卵泡（大于或等于7毫升）中），卵母细胞的空泡化程度与α1 - 抗胰蛋白酶水平呈负相关，而细胞器聚集程度与蛋白聚糖和免疫球蛋白水平相关。在该样本中，只有五个卵母细胞（15%）具有与正常妊娠潜力相符的卵泡特征。这些处于第二次减数分裂中期的卵母细胞处于中等体积范围内，空泡化水平低，细胞器聚集程度低。相比之下，基于其卵泡生物化学特征而卵裂潜力低的那些卵母细胞，显示出高细胞质空泡化水平。
