用于定量检测结核病患者中分枝杆菌PstS1的实时免疫PCR技术的开发。
Development of real-time immuno-PCR for the quantitative detection of mycobacterial PstS1 in tuberculosis patients.
作者信息
Sharma Suman, Raj Ankush, Singh Netrapal, Dahiya Bhawna, Sheoran Abhishek, Gupta Krishna B, Mehta Promod K
机构信息
Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.
Department of Statistics, Amity Institute of Applied Sciences, Amity University, Noida 201303, India.
出版信息
J Microbiol Methods. 2017 Jan;132:134-138. doi: 10.1016/j.mimet.2016.12.006. Epub 2016 Dec 7.
A novel indirect real-time immuno-polymerase chain reaction (RT-I-PCR) assay, an evolution of I-PCR, was developed for the quantitative detection of Mycobacterium tuberculosis PstS1 (Rv0934) with a wide dynamic range of 10ng/mL to 1pg/mL in body fluids of tuberculosis (TB) patients, which may monitor the dynamics of disease.
一种新型间接实时免疫聚合酶链反应（RT-I-PCR）检测方法，作为免疫聚合酶链反应（I-PCR）的改进，被开发用于定量检测结核分枝杆菌PstS1（Rv0934），其在结核病（TB）患者体液中的动态检测范围为10ng/mL至1pg/mL，这可能有助于监测疾病动态。
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