Newbould M J, Malam J, McIllmurray J M, Morris J A, Telford D R, Barson A J
Department of Pathology, Lancaster Moor Hospital.
J Clin Pathol. 1989 Sep;42(9):935-9. doi: 10.1136/jcp.42.9.935.
A polyclonal antiserum to toxic shock syndrome toxin (TSST-1) and a standard immunoperoxidase technique were used on formalin fixed tissues from 50 cases of sudden infant death syndrome (SIDS) to determine if the syndrome was associated with bacterial infection. There was strong specific staining in the renal tubular cells in nine (18%) cases. A similar pattern of staining was seen in three of a series of 50 kidneys selected for comparison from a wide range of necropsy cases. The staining was finely granular within the cytoplasm of proximal convoluted tubular cells and diffuse in tubular cell nuclei. In an attempt to validate the staining pattern the immunoperoxidase technique was also performed on formalin fixed kidneys from rats which had been given intravenous injections of crude bacterial products containing TSST-1. These showed coarse granular cytoplasmic staining in proximal convoluted tubules with some diffuse nuclear staining. This pattern was not seen in controls injected with saline. These results indicate that TSST-1 might have a pathogenic role in some cases of SIDS.
采用针对中毒性休克综合征毒素（TSST-1）的多克隆抗血清和标准免疫过氧化物酶技术，对50例婴儿猝死综合征（SIDS）的福尔马林固定组织进行检测，以确定该综合征是否与细菌感染有关。9例（18%）病例的肾小管细胞出现强特异性染色。从一系列广泛尸检病例中选取50个肾脏作为对照，其中3个出现类似的染色模式。染色在近端曲管细胞胞质内呈细颗粒状，在细胞核内呈弥漫性。为验证染色模式，还对静脉注射含TSST-1的粗制细菌产物的大鼠福尔马林固定肾脏进行了免疫过氧化物酶技术检测。这些肾脏近端曲管出现粗颗粒状胞质染色，部分细胞核呈弥漫性染色。注射生理盐水的对照组未见此模式。这些结果表明，TSST-1在某些SIDS病例中可能具有致病作用。
