Hamadto H A, Kamal K A
J Egypt Soc Parasitol. 1989 Dec;19(2 Suppl):921-5.
We describe a dot enzyme-linked immunosorbent assay (dot-ELISA) as a field applicable tool for the rapid diagnosis of schistosomiasis. This antibody capture assay is performed using 50 ng protein of crude Schistosoma mansoni egg antigen (SEA) in 1 microliter volumes per dot. Sera (1 microliter/dot) at 1:40 dilution were optimal. Dot-ELISA results were completely comparable to micro-ELISA.
我们描述了一种斑点酶联免疫吸附测定法（dot-ELISA），作为一种可用于现场快速诊断血吸虫病的工具。这种抗体捕获测定法是在每个斑点中使用1微升含50纳克曼氏血吸虫虫卵粗抗原（SEA）的蛋白质来进行的。血清以1:40稀释（1微升/斑点）时效果最佳。Dot-ELISA的结果与微量ELISA完全可比。
