Alkarmi T, Ali-Khan Z
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
J Parasitol. 1989 Oct;75(5):711-9.
Alveolar hydatid cyst antigen (AHCA) absorbed with Sepharose-coupled anti-mouse Ig or its Sephacryl S-300 fractions was assayed for phlogistic/chemotactic and amyloidogenic properties in C57BL/6J mice. The in vivo and in vitro biological properties of the antigen were assessed by intradermal or intraperitoneal routes in mice or in Boyden chambers, respectively. In both these assays the chemotactic activity of the antigen was found to be dose dependent. A single intradermal injection of the antigen, containing 35 or 70 micrograms protein, showed a peak inflammatory cell response in the dermal layers at 6 hr. Neutrophils were the dominant cellular infiltrates and the number of monocytoid cells, except at 24 hr, remained relatively low. Antigen concentrations ranging from 1 to 200 micrograms protein per Boyden chamber showed peak neutrophilic and monocytoid cell responses with 100 micrograms of the antigen. We therefore conclude that intense inflammatory response and amyloidogenesis in alveolar hydatid cyst-infected murine hosts are directly attributable to the parasite antigen.
用偶联琼脂糖的抗小鼠Ig或其Sephacryl S - 300级分吸收的肺泡包虫囊肿抗原（AHCA），在C57BL / 6J小鼠中检测其致炎/趋化和淀粉样变特性。分别通过小鼠皮内或腹腔途径或在博伊登小室中评估抗原的体内和体外生物学特性。在这两种测定中，均发现抗原的趋化活性呈剂量依赖性。单次皮内注射含35或70微克蛋白质的抗原，在6小时时真皮层出现炎症细胞反应高峰。中性粒细胞是主要的细胞浸润成分，除24小时外，单核样细胞数量相对较低。每个博伊登小室中抗原浓度为1至200微克蛋白质时，100微克抗原显示出中性粒细胞和单核样细胞反应高峰。因此，我们得出结论，肺泡包虫囊肿感染的鼠宿主中的强烈炎症反应和淀粉样变直接归因于寄生虫抗原。
