Qi S B, Kitamura Y, Nomura Y
Department of Pharmacology, Toyama Medical and Pharmaceutical University, Japan.
J Pharmacobiodyn. 1989 Apr;12(4):216-9. doi: 10.1248/bpb1978.12.216.
The effects of cholera and pertussis toxin on the release of noradrenaline (NA) were studied in the rat cerebral cortex. The cerebral cortical slices were incubated with cholera or pertussis toxin for 2 h, subsequently loaded with 1-[3H]NA and superfused continuously. The pretreatment with cholera toxin significantly (p less than 0.05) enhanced the 20 mM KCl-evoked [3H]NA release. In contrast to a significant (p less than 0.05) increase in [3H]NA release by pertussis toxin, neither A-protomer nor B-oligomer of the toxin could affect the release. These results suggest that cholera and pertussis toxin-substrates, probably guanosine triphosphate-binding proteins, could be involved in the NA release in the central nervous system.
在大鼠大脑皮层中研究了霍乱毒素和百日咳毒素对去甲肾上腺素（NA）释放的影响。将大脑皮层切片与霍乱毒素或百日咳毒素孵育2小时，随后加载1-[³H]NA并持续进行灌流。用霍乱毒素预处理显著（p<0.05）增强了20mM氯化钾诱发的[³H]NA释放。与百日咳毒素使[³H]NA释放显著（p<0.05）增加相反，该毒素的A亚基和B寡聚体均不影响释放。这些结果表明，霍乱毒素和百日咳毒素的底物，可能是鸟苷三磷酸结合蛋白，可能参与中枢神经系统中的NA释放。
