Kawata K, Nomura Y
Neurosci Res. 1987 Feb;4(3):236-40. doi: 10.1016/0168-0102(87)90015-0.
Clonidine at 1.0 microM significantly decreased 20 mM K+-evoked release of L-[3H]noradrenaline (NA) from rat cerebral cortical slices preloaded with L-[3H]NA. Inhibitory effects of clonidine, however, were not observed in slices pretreated with 20 micrograms/ml pertussis toxin, an islet-activating protein, together with NAD and adenosine triphosphate. It is suggested that the inhibitory guanine nucleotide-binding protein (Ni) could be involved in alpha 2-adrenoceptor-mediated inhibition of NA release from nerve terminals in the central nervous system.
1.0微摩尔的可乐定显著降低了预先用L-[3H]去甲肾上腺素（NA）加载的大鼠大脑皮层切片中20毫摩尔钾离子诱发的L-[3H]去甲肾上腺素（NA）释放。然而，在用20微克/毫升百日咳毒素（一种胰岛激活蛋白）与烟酰胺腺嘌呤二核苷酸（NAD）和三磷酸腺苷（ATP）一起预处理的切片中未观察到可乐定的抑制作用。提示抑制性鸟嘌呤核苷酸结合蛋白（Ni）可能参与中枢神经系统中α2肾上腺素能受体介导的神经末梢NA释放的抑制。
