脑反应性抗体与艾滋病痴呆综合征

Brain-reactive antibodies and the AIDS dementia complex.

作者信息

Kumar M, Resnick L, Loewenstein D A, Berger J, Eisdorfer C

机构信息

Department of Psychiatry, University of Miami, School of Medicine, FL 33101.

出版信息

J Acquir Immune Defic Syndr (1988). 1989;2(5):469-71.

PMID:2795448

Abstract

To determine the role of autoantibodies in the development of the AIDS dementia complex, brain-reactive antibodies (BRAs) were assayed in 30 sera samples obtained from AIDS patients. Eighteen samples were obtained from AIDS patients with neuropsychological complications, and 12 samples were obtained from patients without any neuropsychological involvement. BRAs were analyzed using strips of nitrocellulose electroblots as a source of antigen, and were prepared from a sample of normal human parahippocampal tissue subjected to SDS-PAGE. Fourteen of 18 patients with neuropsychological complications and 4 of 12 patients without such complications were positive for BRAs to a protein with Mr of 45,000-50,000.

摘要

为了确定自身抗体在艾滋病痴呆综合征发展中的作用，对30份来自艾滋病患者的血清样本检测了脑反应性抗体（BRA）。18份样本取自伴有神经心理学并发症的艾滋病患者，12份样本取自无任何神经心理学病变的患者。采用硝酸纤维素电印迹条作为抗原来源分析BRA，抗原由经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）的正常人海马旁回组织样本制备。18例有神经心理学并发症的患者中有14例，12例无此类并发症的患者中有4例，其针对分子量为45,000 - 50,000的蛋白质的BRA呈阳性。