Jensen Louise Kruse, Koch Janne, Dich-Jorgensen Kirstine, Aalbaek Bent, Petersen Andreas, Fuursted Kurt, Bjarnsholt Thomas, Kragh Kasper Nørskov, Tøtterup Mikkel, Bue Mats, Hanberg Pelle, Søballe Kjeld, Heegaard Peter M H, Jensen Henrik Elvang
Department of Veterinary Disease Biology, University of Copenhagen, Ridebanevej 3, 1870, Frederiksberg C, Denmark.
Department of Experimental Medicine, University of Copenhagen, Blegdamsvej 2, 2200, Copenhagen N, Denmark.
J Orthop Res. 2017 Oct;35(10):2211-2221. doi: 10.1002/jor.23505. Epub 2017 Jan 15.
Pigs are favorable experimental animals for infectious diseases in humans. However, implant-associated osteomyelitis (IAO) models in pigs have only been evaluated using high-inoculum infection (>10 CFU) models in 1975 and 1993. Therefore, the aim of this paper was to present a new low inoculum porcine model of human IAO based on 42 experimental pigs. The model was created by drilling an implant cavity in the tibial bone followed by insertion of a small steel implant and simultaneous inoculation of Staphylococcus aureus bacteria (n = 32) or saline (n = 10). The infected pigs were either inoculated with 10 CFU (n = 26) or 10 and 10 CFU (n = 6). All animals were euthanized 5 days after insertion of implants. Pigs receiving the high-inoculum infections showed a significantly higher volume of bone lesion, number of neutrophils around the implant, concentrations of acute phase proteins in serum, and enlargement of regional lymph nodes. A positive correlation was present between a high number of surrounding neutrophils and high values of all other parameters. Furthermore, a threshold of 40 neutrophils per 10 high power fields for the histopathological diagnosis of high grade IAO was defined.
This paper describes a novel low-inoculum S. aureus porcine model of IAO which was demonstrated to be reliable, reproducible and discriminative to human IAO, and represents a requested and valuable tool in orthopedic research. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2211-2221, 2017.
猪是研究人类传染病的理想实验动物。然而，猪的植入物相关骨髓炎（IAO）模型仅在1975年和1993年使用高接种量感染（>10 CFU）模型进行过评估。因此，本文的目的是基于42只实验猪提出一种新的低接种量人类IAO猪模型。该模型通过在胫骨上钻出植入腔，然后插入一个小钢植入物并同时接种金黄色葡萄球菌（n = 32）或生理盐水（n = 10）来创建。感染的猪要么接种10 CFU（n = 26），要么接种10和10 CFU（n = 6）。所有动物在植入物插入5天后实施安乐死。接受高接种量感染的猪表现出明显更大的骨病变体积、植入物周围的中性粒细胞数量、血清中急性期蛋白的浓度以及区域淋巴结肿大。大量周围中性粒细胞与所有其他参数的高值之间存在正相关。此外，定义了每10个高倍视野40个中性粒细胞的阈值用于高级别IAO的组织病理学诊断。
本文描述了一种新型的低接种量金黄色葡萄球菌猪IAO模型，该模型被证明对人类IAO可靠、可重复且具有鉴别性，是骨科研究中一种急需且有价值的工具。© 2016骨科研究协会。由威利期刊公司出版。《矫形外科学研究》35:2211 - 2221，2017年。
