Ohhira S, Matsui H
Department of Hygiene, Dokkyo University School of Medicine.
Sangyo Igaku. 1989 May;31(3):150-5. doi: 10.1539/joh1959.31.150.
Determination of tributyltin and its metabolites, dibutyltin and monobutyltin, in biological materials was made by capillary gas chromatography (C-GC) using a flame photometric detector (FPD). Butyltin compounds (BuTC) were extracted (as bromides) from tissue homogenates with hydrobromic acid and ethyl acetate. These compounds were converted to pentyl derivatives with pentyl Grignard reagent and then analysed by C-GC. The recoveries of each BuTC added to tissues were 96-99% for monobutyltin, 87-93% for dibutyltin and 90-93% for tributyltin. The detection limit of BuTC was 4-5 pg as tin. This method was applied to the analysis of BuTC in the liver and kidney of rats orally administered tributyltin chloride. Time course of three BuTC showed that the maximum value appeared 24 h after administration of the tin compound, which was followed by a rapid decrease. The order of the concentration of BuTC in both organs was dibutyltin greater than tributyltin greater than monobutyltin. The rate of dealkylation was more rapid in liver than in kidney.
采用配备火焰光度检测器（FPD）的毛细管气相色谱法（C-GC）测定生物材料中的三丁基锡及其代谢产物二丁基锡和单丁基锡。用氢溴酸和乙酸乙酯从组织匀浆中提取（以溴化物形式）丁基锡化合物（BuTC）。这些化合物用戊基格氏试剂转化为戊基衍生物，然后通过C-GC进行分析。添加到组织中的每种BuTC的回收率分别为：单丁基锡96 - 99%，二丁基锡87 - 93%，三丁基锡90 - 93%。BuTC的检测限以锡计为4 - 5 pg。该方法应用于口服三丁基氯化锡的大鼠肝脏和肾脏中BuTC的分析。三种BuTC的时间进程显示，在给予锡化合物后24小时出现最大值，随后迅速下降。两个器官中BuTC的浓度顺序为二丁基锡大于三丁基锡大于单丁基锡。肝脏中的脱烷基化速率比肾脏中更快。
