S S Sarayu Gopal, Pillai Agieshkumar B, Ramachandrappa Vijayakumar Shettikothanuru, T Kadhiravan, Dhodapkar Rahul, Kah James, Rajendiran Soundravally
Department of Biochemistry, JIPMER, Puducherry, India.
Central Inter-Disciplinary Research Facility (CIDRF), A Unit of Sri Balaji Educational and Charitable Public Trust, SBVU, Puducherry, India.
J Clin Virol. 2017 Jan;86:62-67. doi: 10.1016/j.jcv.2016.10.009. Epub 2016 Nov 17.
Dengue viruses are known to infect and replicate in macrophages. Thus studying the host responsive molecules that are specifically released by macrophages during the course of dengue infection may provide better understanding on dengue immunopathogenesis. Soluble CD163 (sCD163) is a scavenger receptor, highly expressed on macrophages reported to be involved in some viral disease. The participation of sCD163 in dengue is not known.
The present study aimed to explore the role of sCD163 as a potential biomarker for predicting dengue disease.
Using a case-control design, 82 dengue subjects consisting of 69 non-severe dengue (NSD) and 13 severe dengue (SD) along with 32 non-dengue other febrile illness (OFI) subjects and 30 healthy subjects were involved in the study. The serum concentration of sCD163 was determined in the study subjects at admission and around defervescence using ELISA. Statistical analysis was done using Mann-Whitney U test andWilcoxon signed rank test.
The study recorded a significant increase in the sCD163 serum level at defervescence phase specifically among dengue group compared to OFI. sCD163 was also found to be significantly higher in secondary cases compared to primary at both admission and defervescence. Furthermore,a higher level of sCD163 was also observed in SD compared to NSD cases, although no statistical significance was observed CONCLUSION: The study substantiates the role of macrophage activation in dengue pathogenesis and further study is needed to decipher the exact role of sCD163 in the disease pathogenesis and to explore its potential as a marker for the early prediction of dengue severity.
已知登革病毒可在巨噬细胞中感染并复制。因此，研究登革热感染过程中巨噬细胞特异性释放的宿主反应性分子，可能有助于更好地理解登革热免疫发病机制。可溶性CD163（sCD163）是一种清道夫受体，在巨噬细胞上高度表达，据报道与某些病毒性疾病有关。sCD163在登革热中的作用尚不清楚。
本研究旨在探讨sCD163作为预测登革热疾病潜在生物标志物的作用。
采用病例对照设计，82名登革热患者，包括69例非重症登革热（NSD）和13例重症登革热（SD），以及32例非登革热的其他发热性疾病（OFI）患者和30名健康受试者参与了研究。采用酶联免疫吸附测定法（ELISA）测定研究对象入院时和退热期前后血清中sCD163的浓度。采用Mann-Whitney U检验和Wilcoxon符号秩检验进行统计学分析。
研究记录显示，与OFI组相比，登革热组在退热期血清sCD163水平显著升高。在入院时和退热期，继发性病例的sCD163也显著高于原发性病例。此外，与NSD病例相比，SD病例中也观察到较高水平的sCD163，尽管未观察到统计学意义。结论：该研究证实了巨噬细胞激活在登革热发病机制中的作用，需要进一步研究以阐明sCD163在疾病发病机制中的具体作用，并探索其作为登革热严重程度早期预测标志物的潜力。
