Poobathy Ranjetta, Zakaria Rahmad, Hamzah Syed Mohd Edzham Syed, Subramaniam Sreeramanan
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Trop Life Sci Res. 2016 Nov;27(supp1):17-19. doi: 10.21315/tlsr2016.27.3.3.
The terrestrial , also referred to as the jewel orchid is prized for the quality of its leaves. is known as a medicinal herb and is touted for its heat- and pathogen-resisting qualities. is valuable in the production of both flavonoids and anthocyanins, antioxidants that are exalted in the health industry. Plant cell cultures have emerged as alternative sources of anthocyanin production. Plant protoplast cultures are used frequently in transient gene expression studies and in the establishment of callus and cell suspension cultures. Benefits of plant protoplast system include similarity to cells found in plant tissues, reproduction under controlled conditions, and prevention of masking of stress responses to previous handling techniques. A study was conducted to assess the amenability of the stem and leaves of to protoplast isolation. The stem and leaf segments were weighed, sliced into thin layers, immersed in a digestion medium, washed and then cultured onto a recovery medium. Results indicated that the production of plant protoplasts from may be viewed as an alternative in the generation of cell cultures and ultimately in the production of anthocyanins from the cell cultures.
地生植物，也被称为宝石兰，因其叶片的品质而备受珍视。它作为一种药草而闻名，并因其耐热和抗菌特性而受到推崇。它在黄酮类化合物和花青素的生产中很有价值，花青素是健康产业中备受赞誉的抗氧化剂。植物细胞培养已成为花青素生产的替代来源。植物原生质体培养常用于瞬时基因表达研究以及愈伤组织和细胞悬浮培养的建立。植物原生质体系统的优点包括与植物组织中的细胞相似、在可控条件下繁殖以及防止对先前处理技术的应激反应被掩盖。进行了一项研究以评估[植物名称未明确]的茎和叶对原生质体分离的适宜性。将茎和叶段称重，切成薄片，浸入消化培养基中，洗涤后再接种到恢复培养基上。结果表明，从[植物名称未明确]生产植物原生质体可被视为细胞培养生成以及最终从细胞培养物中生产花青素的一种替代方法。
