使用设计的DNA载体和固态纳米孔进行基于单分子的单核苷酸多态性检测。
Single molecule based SNP detection using designed DNA carriers and solid-state nanopores.
作者信息
Kong Jinglin, Zhu Jinbo, Keyser Ulrich F
机构信息
Cavendish Laboratory, University of Cambridge, Cambridge, UK.
出版信息
Chem Commun (Camb). 2016 Dec 22;53(2):436-439. doi: 10.1039/c6cc08621g.
PMID:27965988
Abstract
Single nucleotide polymorphisms (SNPs) play a crucial role as molecular markers in medical and diagnostic application. We demonstrate a nanopore based method for SNP detection at the single molecule level. Designed DNA carriers are used to distinguish DNA strands containing only one single base difference and follow strand displacement kinetics.
摘要
单核苷酸多态性（SNPs）作为分子标记物在医学和诊断应用中发挥着关键作用。我们展示了一种基于纳米孔的单分子水平SNP检测方法。设计的DNA载体用于区分仅含一个单碱基差异的DNA链，并追踪链置换动力学。
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