Cavendish Laboratory, University of Cambridge, JJ Thompson Avenue, Cambridge CB3 0HE, United Kingdom.
Nano Lett. 2022 Jun 22;22(12):4993-4998. doi: 10.1021/acs.nanolett.2c01764. Epub 2022 Jun 7.
Assembly of DNA structures based on hybridization like split G-quadruplex (GQ) have great potential for the base-pair specific identification of nucleic acid targets. Herein, we combine multiple split G-quadruplex (GQ) assemblies on designed DNA nanostructures (carrier) with a solid-state nanopore sensing platform. The split GQ probes recognize various nucleic acid sequences in a parallel assay that is based on glass nanopore analysis of molecular structures. Specifically, we split a GQ into two asymmetric parts extended with sequences complementary to the target. The longer G-segment is in solution, and the shorter one is on a DNA carrier. If the target is present, the two separate GQ parts will be brought together to facilitate the split GQ formation and enhance the nanopore signal. We demonstrated detection of multiple target sequences from different viruses with low crosstalk. Given the programmability of this DNA based nanopore sensing platform, it is promising in biosensing.
基于杂交的 DNA 结构组装，如分裂 G-四链体 (GQ)，在碱基对特异性识别核酸靶标方面具有很大的潜力。在此，我们将多个设计的 DNA 纳米结构（载体）上的分裂 G-四链体 (GQ) 组装与固态纳米孔传感平台相结合。分裂 GQ 探针在基于玻璃纳米孔分析分子结构的平行测定中识别各种核酸序列。具体来说，我们将 GQ 分裂成两个不对称的部分，延伸的序列与靶标互补。较长的 G 片段在溶液中，较短的片段在 DNA 载体上。如果存在靶标，两个单独的 GQ 部分将被聚集在一起，以促进分裂 GQ 的形成并增强纳米孔信号。我们证明了可以从不同病毒中检测到多个目标序列，并且具有低串扰。鉴于这种基于 DNA 的纳米孔传感平台的可编程性，它在生物传感方面具有广阔的前景。
