State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Microb Ecol. 2022 Jan;83(1):151-166. doi: 10.1007/s00248-021-01715-w. Epub 2021 Mar 23.
Symbioses between invasive insects and bacteria are one of the key drivers of insect invasion success. Gall-inducing insects stimulate host plants to produce galls, which affects the normal growth of plants. Leptocybe invasa Fisher et La Salle, an invasive gall-inducing wasp, mainly damages Eucalyptus plantations in Southern China, but little is known about its associated bacteria. The aim of this study was to assess the diversity of bacterial communities at different developmental stages of L. invasa and to identify possible ecological functions of the associated bacteria. Bacteria associated with L. invasa were isolated using culture-dependent methods and their taxonomic statuses were determined by sequencing the 16S rRNA gene. A total of 88 species belonging to four phyla, 27 families, and 44 genera were identified by phylogenetic analysis. The four phyla were Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes, mainly from the genera Pantoea, Enterobacter, Pseudomonas, Bacillus, Acinetobacter, Curtobacterium, Sphingobium, Klebsiella, and Rhizobium. Among them, 72 species were isolated in the insect gall stage and 46 species were isolated from the adult stage. The most abundant bacterial species were γ-Proteobacteria. We found significant differences in total bacterial counts and community compositions at different developmental stages, and identified possible ecological roles of L. invasa-associated bacteria. This study is the first to systematically investigate the associated bacteria of L. invasa using culture-dependent methods, and provides a reference for other gall-inducing insects and associated bacteria.
共生关系是昆虫成功入侵的关键驱动因素之一。诱导瘿的昆虫会刺激宿主植物产生瘿，从而影响植物的正常生长。入侵性瘿蜂 Leptocybe invasa Fisher et La Salle 主要破坏中国南方的桉树种植园，但对其相关细菌知之甚少。本研究旨在评估 L. invasa 在不同发育阶段的细菌群落多样性，并确定相关细菌的可能生态功能。采用基于培养的方法分离与 L. invasa 相关的细菌，并通过测序 16S rRNA 基因确定其分类地位。通过系统发育分析共鉴定出 88 个物种，属于 4 个门、27 个科和 44 个属。这四个门是变形菌门、放线菌门、厚壁菌门和拟杆菌门，主要来自泛菌属、肠杆菌属、假单胞菌属、芽孢杆菌属、不动杆菌属、短小杆菌属、鞘氨醇单胞菌属、克雷伯氏菌属和根瘤菌属。其中，72 种在昆虫瘿阶段分离，46 种从成虫阶段分离。最丰富的细菌物种是γ-变形菌。我们发现不同发育阶段的总细菌计数和群落组成存在显著差异，并确定了 L. invasa 相关细菌的可能生态作用。本研究首次采用基于培养的方法系统研究了 L. invasa 的相关细菌，为其他诱导瘿的昆虫及其相关细菌提供了参考。
