Identification of HIV-1 infected seropositive subjects by a direct diagnostic test involving hybridization of amplified viral DNA.

We describe a simple slot-blot hybridization procedure with an oligo-probe for identification of amplified HIV-1 DNA in seropositive subjects. Comparison are realized, on the same DNA samples, with other methods DNA detection including Southern blot tests after hybridization with a labelled probe, and autoradiographic patterns after digestion with a restriction enzyme of the amplified product hybridized. This new direct approach towards diagnosis of HIV-1 infection easily be carried out on a large scale.