Loche M, Mach B
Department of Microbiology, University of Geneva Medical School, Switzerland.
Lancet. 1988 Aug 20;2(8608):418-21. doi: 10.1016/s0140-6736(88)90412-6.
There is a need for direct detection of the virus in people infected with human immunodeficiency virus (HIV), independently of a serological response. In this study, after enzymic amplification of a specific segment of the HIV genome, a simple slot-blot hybridisation procedure allowed unequivocal identification of HIV DNA in all seropositive subjects tested. More importantly, the hybridisation test allowed the detection of HIV DNA in several seronegative subjects from very high risk groups. This new direct approach towards the diagnosis of HIV infection, which can easily be carried out on a large scale, is therefore capable of identifying HIV-infected individuals before the development of antibodies.
需要在感染人类免疫缺陷病毒（HIV）的人群中直接检测病毒，而不依赖于血清学反应。在本研究中，对HIV基因组的特定片段进行酶促扩增后，一种简单的斑点杂交程序能够明确鉴定所有检测的血清阳性受试者中的HIV DNA。更重要的是，杂交试验能够在几个来自高危人群的血清阴性受试者中检测到HIV DNA。因此，这种易于大规模开展的新型HIV感染诊断直接方法能够在抗体产生之前识别出HIV感染个体。
