[使用高效液相色谱法测定血浆中25-羟基维生素D3的方法学建议]
[Methodologic recommendations for the determination of 25-OH vitamin D3 in plasma using HPLC].
作者信息
Matthey M, Graf H, Richter G H, Flachowsky G
机构信息
Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig, Wissenschaftsbereich Tierernährungschemie DDR.
出版信息
Nahrung. 1989;33(6):503-7.
A method for measuring 25-OH D3-vitamin (calcidiol) in plasma samples of cattle is described. The samples were purified using non-polar C 18-cartridges and 25-OH D3 was determined by high-performance liquid chromatography (HPLC) on a silicic acid straight phase liquid chromatography column (retention time 11.5 min). The detection limit for 25-OH D3 is about 0.5 ng injection quantity, the average recovery was 90%, the standard error amounted to 8%.
本文描述了一种测定牛血浆样本中25-羟基维生素D3（骨化二醇）的方法。样本用非极性C18柱进行纯化，然后在硅酸正相液相色谱柱上通过高效液相色谱法（HPLC）测定25-羟基维生素D3（保留时间11.5分钟）。25-羟基维生素D3的检测限约为0.5 ng进样量，平均回收率为90%，标准误差为8%。
Zotero 插件