单克隆抗体对传染性胃肠炎病毒的补体依赖中和作用
Complement-dependent neutralization of transmissible gastroenteritis virus by monoclonal antibodies.
作者信息
Woods R D, Wesley R D, Kapke P A
机构信息
USDA-ARS, National Animal Disease Center, Ames, Ia.
出版信息
Adv Exp Med Biol. 1987;218:493-500. doi: 10.1007/978-1-4684-1280-2_64.
Monoclonal antibodies (MAb) to each of the 3 major structural proteins of transmissible gastroenteritis virus (TGEV) of swine were compared as to their virus neutralizing activity in the presence or absence of guinea pig complement. MAbs to the peplomer protein had neutralizing activity for TGEV with or without complement and the titers were similar in either case. MAbs to the matrix protein had neutralizing activity for TGEV only in the presence of complement. Antibodies to the nucleocapsid protein were without neutralizing activity with or without complement. High concentrations of guinea pig complement, but not swine complement, had neutralizing activity for TGEV even in the absence of any known TGEV antibodies.
比较了针对猪传染性胃肠炎病毒（TGEV）三种主要结构蛋白的单克隆抗体（MAb）在有或没有豚鼠补体存在时的病毒中和活性。针对纤突蛋白的单克隆抗体在有或没有补体的情况下对TGEV都具有中和活性，且两种情况下的效价相似。针对基质蛋白的单克隆抗体仅在有补体存在时对TGEV具有中和活性。针对核衣壳蛋白的抗体无论有无补体都没有中和活性。即使在没有任何已知TGEV抗体的情况下，高浓度的豚鼠补体（而非猪补体）对TGEV也具有中和活性。
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