Li Xiaodan, Yu Xiaoyan, Sun Dewei, Li Jinwei, Wang Yong, Cao Peirang, Liu Yuanfa
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University , Wuxi 214122, China.
Department of Food Science and Engineering, Jinan University , Guangzhou 510632, China.
J Agric Food Chem. 2017 Jan 11;65(1):208-215. doi: 10.1021/acs.jafc.6b04565. Epub 2016 Dec 23.
In the present study, effects of deep-fried palm oil, specifically polar compounds generated during the frying process, on animal health including lipid and glucose metabolism and liver functions were investigated. Kunming mice were fed a high-fat diet containing deep-fried palm oil or purified polar compounds for 12 weeks. Their effects on animal health including hepatic lipid profile, antioxidant enzyme activity, serum biochemistry, and glucose tolerance were analyzed. Our results revealed that the consumption of polar compounds was related to the change of lipid deposition in liver and adipose tissue, as well as glucose tolerance alteration in Kunming mice. Correspondingly, the transcription study of genes involved in lipid metabolism including PPARα, Acox1, and Cpt1α indicated that polar compounds probably facilitated the fatty acid oxidation on peroxisomes, whereas lipid oxidation in mitochondria was suppressed. Furthermore, glucose tolerance test (GTT) revealed that a high amount of polar compound intake impaired glucose tolerance, indicating its effect on glucose metabolism in vivo. Our results provide critical information on the effects of polar compounds generated from the deep-frying process of palm oil on animal health, particularly liver functions and lipid and glucose metabolism, which is important for the evaluation of the biosafety of frying oil.
在本研究中，我们调查了深度油炸棕榈油，特别是油炸过程中产生的极性化合物，对动物健康（包括脂质和葡萄糖代谢以及肝功能）的影响。将昆明小鼠喂食含深度油炸棕榈油或纯化极性化合物的高脂饮食12周。分析了它们对动物健康的影响，包括肝脏脂质谱、抗氧化酶活性、血清生化指标和葡萄糖耐量。我们的结果显示，极性化合物的摄入与昆明小鼠肝脏和脂肪组织中脂质沉积的变化以及葡萄糖耐量改变有关。相应地，对参与脂质代谢的基因（包括PPARα、Acox1和Cpt1α）的转录研究表明，极性化合物可能促进了过氧化物酶体上的脂肪酸氧化，而线粒体中的脂质氧化则受到抑制。此外，葡萄糖耐量试验（GTT）显示，大量摄入极性化合物会损害葡萄糖耐量，表明其对体内葡萄糖代谢有影响。我们的结果提供了关于棕榈油油炸过程中产生的极性化合物对动物健康，特别是肝功能以及脂质和葡萄糖代谢影响的关键信息，这对于评估煎炸油的生物安全性很重要。
