Kaitetzidou Elisavet, Ludwig Arne, Gessner Jörn, Sarropoulou Elena
Institute for Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, Greece.
School of Biology, Faculty of Science, Aristotle University of Thessaloniki, 54124, Greece.
G3 (Bethesda). 2017 Feb 9;7(2):533-542. doi: 10.1534/g3.116.036699.
During teleost ontogeny the larval and embryonic stages are key stages, since failure during this period of tissue differentiation may cause malformations, developmental delays, poor growth, and massive mortalities. Despite the rapid advances in sequencing technologies, the molecular backgrounds of the development of economically important but endangered fish species like the Atlantic sturgeon () have not yet been thoroughly investigated. The current study examines the differential expression of transcripts involved in embryonic development of the Atlantic sturgeon. Addressing this goal, a reference transcriptome comprising eight stages was generated using an Illumina HiSequation 2500 platform. The constructed assembly counted to 441,092 unfiltered and 179,564 filtered transcripts. Subsequently, the expression profile of four developmental stages ranging from early (gastrula) to late stages of prelarval development [2 d posthatching (dph)] were investigated applying an Illumina MiSeq platform. Differential expression analysis revealed distinct expression patterns among stages, especially between the two early and the two later stages. Transcripts upregulated at the two early stages were mainly enriched in transcripts linked to developmental processes, while transcripts expressed at the last two stages were mainly enriched in transcripts important to muscle contraction. Furthermore, important stage-specific expression has been detected for the hatching stage with transcripts enriched in molecule transport, and for the 2 dph stage with transcripts enriched in visual perception and lipid digestion. Our investigation represents a significant contribution to the understanding of Atlantic sturgeon embryonic development, and transcript characterization along with the differential expression results will significantly contribute to sturgeon research and aquaculture.
在硬骨鱼个体发育过程中，幼体和胚胎阶段是关键阶段，因为在此组织分化时期出现问题可能会导致畸形、发育迟缓、生长不良以及大量死亡。尽管测序技术取得了快速进展，但像大西洋鲟鱼（）这样具有经济重要性却濒危的鱼类物种的发育分子背景尚未得到充分研究。本研究考察了大西洋鲟鱼胚胎发育过程中相关转录本的差异表达。为实现这一目标，使用Illumina HiSequation 2500平台构建了一个包含八个阶段的参考转录组。构建的组装体包含441,092条未过滤转录本和179,564条过滤后的转录本。随后，利用Illumina MiSeq平台研究了从早期（原肠胚）到幼体发育后期[孵化后2天（dph）]四个发育阶段的表达谱。差异表达分析揭示了各阶段之间不同的表达模式，尤其是两个早期阶段和两个后期阶段之间。在两个早期阶段上调的转录本主要富集于与发育过程相关的转录本中，而在最后两个阶段表达的转录本主要富集于对肌肉收缩重要的转录本中。此外，已检测到孵化阶段有重要的阶段特异性表达，其转录本富集于分子转运方面，而在2 dph阶段，转录本富集于视觉感知和脂质消化方面。我们的研究对理解大西洋鲟鱼胚胎发育做出了重要贡献，转录本特征分析以及差异表达结果将对鲟鱼研究和水产养殖有显著帮助。
