Kambourova Margarita, Tomova Iva, Boyadzhieva Ivanka, Radchenkova Nadja, Vasileva-Tonkova Evgenia
Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 26, 1113 Sofia, Bulgaria.
Archaea. 2016 Nov 16;2016:7459679. doi: 10.1155/2016/7459679. eCollection 2016.
Recent studies on archaeal diversity in few salterns have revealed heterogeneity between sites and unique structures of separate places that hinder drawing of generalized conclusions. Investigations on the archaeal community composition in P18, the biggest crystallizer pond in Pomorie salterns (PS) (34% salinity), demonstrated unusually high number of presented taxa in hypersaline environment. Archaeal clones were grouped in 26 different operational taxonomic units (OTUs) assigned to 15 different genera from two orders, Halobacteriales and Haloferacales. All retrieved sequences were related to culturable halophiles or unculturable clones from saline (mostly hypersaline) niches. New sequences represented 53.9% of archaeal OTUs. Some of them formed separate branches with 90% similarity to the closest neighbor. Present results significantly differed from the previous investigations in regard to the number of presented genera, the domination of some genera not reported before in such extreme niche, and the identification of previously undiscovered 16S rRNA sequences.
最近对少数盐场古菌多样性的研究揭示了不同地点之间的异质性以及不同地方的独特结构，这阻碍了得出一般性结论。对波莫瑞盐场（PS）最大的结晶池P18（盐度34%）中古菌群落组成的调查表明，在高盐环境中出现的分类单元数量异常之多。古菌克隆被归为26个不同的操作分类单元（OTU），这些OTU属于盐杆菌目和嗜盐嗜铁菌目两个目下的15个不同属。所有检索到的序列都与来自盐生（主要是高盐）生态位的可培养嗜盐菌或不可培养克隆有关。新序列占古菌OTU的53.9%。其中一些形成了与最近邻相似度达90%的独立分支。目前的结果在呈现的属数量、此前未在如此极端生态位中报道过的一些属的主导地位以及新发现的16S rRNA序列的鉴定方面，与之前的调查有显著差异。
