Wu Li-Ling, Wang Hurng-Yi, Chen Pei-Jer
Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Methods Mol Biol. 2017;1540:227-235. doi: 10.1007/978-1-4939-6700-1_19.
Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.
水动力乙肝病毒转染小鼠模型是过去十年建立的一种方法，可将通常不能透过细胞膜的大分子递送至细胞内。其基本原理是将含有乙肝病毒质粒构建体的大量溶液快速注入循环系统，以使这些大分子优先进入肝实质细胞。本章旨在描述小鼠模型中水动力乙肝病毒转染的基本原理。
