Kim Young Uk, Kim Hyeon Ji, Kim Eunjeong
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea; School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea; Center for Genome Engineering, Institute for Basic Science, Daejeon 34126, Republic of Korea.
STAR Protoc. 2025 May 3;6(2):103812. doi: 10.1016/j.xpro.2025.103812.
Diesel particulate matter (DPM) is known to infiltrate the body and circulate through the bloodstream, adversely affecting human health. However, liver-specific studies assessing DPM exposure and its effects remain limited. Here, we present a protocol for assessing the effect of DPM on the liver in vivo through hydrodynamic tail vein injection in mice. We describe steps for RNA extraction from liver tissue post-DPM administration, followed by reverse-transcription quantitative PCR (RT-qPCR) to observe changes in gene expression levels.
已知柴油颗粒物（DPM）会侵入人体并在血液循环，对人类健康产生不利影响。然而，评估DPM暴露及其影响的肝脏特异性研究仍然有限。在此，我们提出了一种通过小鼠尾静脉流体动力学注射来评估DPM对肝脏体内影响的方案。我们描述了在给予DPM后从肝脏组织中提取RNA的步骤，随后进行逆转录定量PCR（RT-qPCR）以观察基因表达水平的变化。
