Protocol for assessing the effect of diesel particulate matter on the liver in vivo through hydrodynamic tail vein injection in mice.

Diesel particulate matter (DPM) is known to infiltrate the body and circulate through the bloodstream, adversely affecting human health. However, liver-specific studies assessing DPM exposure and its effects remain limited. Here, we present a protocol for assessing the effect of DPM on the liver in vivo through hydrodynamic tail vein injection in mice. We describe steps for RNA extraction from liver tissue post-DPM administration, followed by reverse-transcription quantitative PCR (RT-qPCR) to observe changes in gene expression levels.