Tang Zuohua, Wu Lingjie, Xiao Zebin, Feng Xiaoyuan, Sun Xinghuai, Tang Weijun, Wang Jie, Jin Lixin
Department of Radiology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
Department of Otolaryngology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
NMR Biomed. 2017 Feb;30(2). doi: 10.1002/nbm.3677. Epub 2016 Dec 15.
This study aimed to explore the optimal dose and manner of administration for visualization of the auditory pathway on manganese-enhanced MRI (ME MRI). Twenty-four healthy male Sprague-Dawley rats were randomly divided into three experimental groups (n = 8 for Groups A, B and C). The rats in Groups A, B and C were subjected to MnCl injection through the tympanum, inner ear endolymph and perilymph, respectively (0.2 M for four rats and 0.4 M for the others in each group) and observed at 1, 2, 3, 4, 7 and 10 days after the operation with 3.0 T MRI. The signal intensity (SI) and dynamic changes of the auditory pathways at various times, and at two doses through three injection routes, were compared by statistical analysis. Administration of MnCl through the perilymph best showed the complete auditory pathway (P < 0.01), whereas administration though the tympanum only demonstrated part of the pathway. The SI was highest at 24 h after administration of the tracer and began to decline at 48 h. The SI of the auditory cortex was higher after the injection of 0.4 M MnCl than that of 0.2 M MnCl . ME MRI best demonstrated the whole auditory pathway at 24 h after the injection of 0.4 M MnCl through the perilymph in the rat, which provided an optimal method for the study of ME MRI of the auditory pathway in the animal model.
本研究旨在探索锰增强磁共振成像（ME MRI）中可视化听觉通路的最佳剂量和给药方式。将24只健康雄性Sprague-Dawley大鼠随机分为三个实验组（A、B、C组每组n = 8）。A、B、C组大鼠分别通过鼓膜、内耳内淋巴和外淋巴注射MnCl（每组4只大鼠注射0.2M，其余注射0.4M），并在术后1、2、3、4、7和10天用3.0T MRI进行观察。通过统计分析比较不同时间、两种剂量以及三种注射途径下听觉通路的信号强度（SI）和动态变化。通过外淋巴注射MnCl最能完整显示听觉通路（P < 0.01），而通过鼓膜注射仅能显示部分通路。示踪剂给药后24小时SI最高，48小时开始下降。注射0.4M MnCl后听觉皮层的SI高于注射0.2M MnCl后。在大鼠中通过外淋巴注射0.4M MnCl后24小时，ME MRI最能完整显示整个听觉通路，这为动物模型中听觉通路的ME MRI研究提供了一种最佳方法。
