Chaves F N, Bezerra Tmm, de Barros Silva P G, Oliveira Faf, Sousa F B, Costa Fwg, Alves Apnn, Pereira Kma
School of Dentistry, Federal University of Ceara/Sobral, Sobral, Ceara, Brazil.
Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Oral Dis. 2017 Apr;23(3):367-378. doi: 10.1111/odi.12623. Epub 2017 Feb 10.
To evaluate the expression of p-AKT, p-JNK, FoxO3a, and Ki-67 in samples of oral squamous cell carcinoma (OSCC) and oral epithelial dysplasias (OEDs) to understand their possible involvement in the malignant transformation process of oral lesions.
Tissue samples of 20 cases of OSCCs, 20 OEDs, and normal oral mucosa were subjected to immunohistochemistry reactions for anti-p-AKT, anti-p-JNK, anti-FoxO3a, and anti-Ki-67 antibodies. It was analyzed using quantitative (number of immunostained cells) and qualitative (immunostaining intensity) parameters in different cell immunostaining sublocations.
Nuclear p-AKT was observed significantly greater immunostaining in OSCC (21.2 ± 19.0) than in dysplasias (7.9 ± 8.1) and controls (1.8 ± 4.7) (P = 0.002). Immunostaining of strong nuclear p-JNK was greater in controls (48.3 ± 13.7) than in OEDs (11.0 ± 10.3) and OSCCs (1.1 ± 1.3) (P < 0.001). Strong nuclear immunostaining of FoxO3a proved to be absent in OSCCs (0.0 ± 0.1) with little staining on dysplasias (3.2 ± 5.4) and increased expression in controls (13.5 ± 4.8) (P < 0.001). Immunostaining of strong nuclear Ki-67 was grater in OSCCs (48.1 ± 49.6) than in OED (11.8 ± 10.6) and controls (1.9 ± 2.0) (P < 0.001).
Malignant process of OEDs in this research may involve the same mechanisms of established malignant lesions.
评估p-AKT、p-JNK、FoxO3a和Ki-67在口腔鳞状细胞癌（OSCC）及口腔上皮发育异常（OED）样本中的表达情况，以了解它们在口腔病变恶性转化过程中可能的作用。
对20例OSCC、20例OED及正常口腔黏膜的组织样本进行抗p-AKT、抗p-JNK、抗FoxO3a和抗Ki-67抗体的免疫组化反应。在不同细胞免疫染色亚定位中，使用定量（免疫染色细胞数量）和定性（免疫染色强度）参数进行分析。
观察到OSCC中核p-AKT的免疫染色（21.2±19.0）显著高于发育异常（7.9±8.1）和对照组（1.8±4.7）（P = 0.002）。强核p-JNK的免疫染色在对照组（48.3±13.7）高于OED（11.0±10.3）和OSCC（1.1±1.3）（P < 0.001）。OSCC中FoxO3a的强核免疫染色缺失（0.0±0.1），发育异常中有少量染色（3.2±5.4），对照组中表达增加（13.5±4.8）（P < 0.001）。强核Ki-67的免疫染色在OSCC（48.1±49.6）高于OED（11.8±10.6）和对照组（1.9±2.0）（P < 0.001）。
本研究中OED的恶性过程可能涉及与已确诊恶性病变相同的机制。
