Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil.
Hospital Haroldo Juaçaba, Ceará Cancer Institute, Fortaleza, Ceará, Brazil.
Asian Pac J Cancer Prev. 2024 Nov 1;25(11):3807-3815. doi: 10.31557/APJCP.2024.25.11.3807.
Squamous cell carcinoma (SCC) is the most common histological type in the oropharynx with high incidence and mortality. The Pi3K-AKT signaling pathway is important for understanding prognostic factors such as metastasis, which is involved in the aetiopathogenesis of cancer and is associated with angiogenesis, progression, and cell survival. The aim of this study was to evaluate the correlation of the Pi3K/AKT pathway with prognostic factors associated with oropharyngeal SCC, including overall survival.
Patients diagnosed and treated for oropharyngeal SCC at the Haroldo Juaçaba Hospital were included in the study. Clinicopathological and socio-demographic data were collected by reviewing patient records and reports. Excisional biopsies were analyzed by immunohistochemical techniques using TMA for the detection of antibodies against ANTI-AKT, ANTI-Pi3K, ANTI-p16, ANTI-Ki67, and ANTI-p53. Immunoexpression was evaluated qualitatively and quantitatively using ImageJ software, and data were correlated with patient survival.
There was a significant reduction in nuclear Pi3K immunoexpression in perilesional tissue compared to primary tumors and nodal metastases (p<0.001). Cytoplasmic Pi3K was increased in primary tumors and decreased in nodal metastases (p=0.001). pAKT expression in p16-negative tumors was associated with poorer overall survival. Further studies are needed to understand how this pathway may influence tumor progression and multimodal therapies.
The PI3K/AKT pathway regulates cell survival and apoptosis and is associated with the malignant transformation of oropharyngeal tumors. AKT expression in p16-negative tumors is a strong predictor of poor prognosis.
鳞状细胞癌（SCC）是口咽部最常见的组织学类型，具有较高的发病率和死亡率。PI3K-AKT 信号通路对于理解转移等预后因素非常重要，它涉及癌症的发病机制，并与血管生成、进展和细胞存活有关。本研究旨在评估 PI3K/AKT 通路与口咽 SCC 相关的预后因素的相关性，包括总生存率。
本研究纳入了在 Haroldo Juaçaba 医院诊断和治疗的口咽 SCC 患者。通过回顾患者的病历和报告收集临床病理和社会人口统计学数据。使用 TMA 对切除活检进行免疫组织化学分析，以检测针对 ANTI-AKT、ANTI-Pi3K、ANTI-p16、ANTI-Ki67 和 ANTI-p53 的抗体。使用 ImageJ 软件对免疫表达进行定性和定量评估，并将数据与患者的生存情况相关联。
与原发性肿瘤和淋巴结转移相比，病变周围组织中的核 PI3K 免疫表达显著降低（p<0.001）。原发性肿瘤中的细胞质 Pi3K 增加，而淋巴结转移中的细胞质 Pi3K 减少（p=0.001）。p16 阴性肿瘤中的 pAKT 表达与总生存率较差相关。需要进一步研究以了解该途径如何影响肿瘤的进展和多模式治疗。
PI3K/AKT 通路调节细胞存活和凋亡，与口咽肿瘤的恶性转化有关。p16 阴性肿瘤中的 AKT 表达是预后不良的强烈预测指标。
