Xie Minhao, Chen Guijie, Hu Bing, Zhou Li, Ou Shiyi, Zeng Xiaoxiong, Sun Yi
College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, China.
Department of Food Science and Engineering, Jinan University , Guangzhou 510632, Guangdong, China.
J Agric Food Chem. 2016 Dec 28;64(51):9624-9630. doi: 10.1021/acs.jafc.6b04710. Epub 2016 Dec 15.
Monocaffeoylquinic acids (mono-CQAs) can be hydrolyzed or metabolized by pancreatin, intestinal brush border esterase, and microbiota in the colon. Data about the conversion of dicaffeoylquinic acids (diCQAs) in digestion are scarce. The diCQA-rich fraction including 3,4-, 3,5-, and 4,5-diCQAs was prepared from Ilex kudingcha, and the conversion in simulated gastricintestine was investigated. Artificial saliva, gastric and pancreatic fluids, Caco-2 monolayer cells, and anaerobic fermentation model were utilized to mimic digestions of the oral cavity, stomach, small intestine, and colon in vitro. The results revealed that diCQAs remained intact in simulated saliva, gastric, and pancreatic fluids and within Caco-2 cells. In anaerobic fermentation with human fecal slurry, diCQAs were hydrolyzed to mono-CQAs and caffeic acid, which were further metabolized to caffeic acid and dihydrocaffeic acid, respectively. The hydrolysis of diCQAs depended on the chemical structures, carbohydrates in the culture medium, and microbial compositions. Our research demonstrated that hydrolysis of diCQAs happened in the colon by intestinal microbiota.
单咖啡酰奎宁酸（单 CQAs）可被胰酶、肠刷状缘酯酶以及结肠中的微生物群水解或代谢。关于二咖啡酰奎宁酸（二 CQAs）在消化过程中转化的数据很少。从苦丁茶中制备了富含 3,4 -、3,5 - 和 4,5 - 二 CQAs 的二 CQA 组分，并研究了其在模拟胃肠道中的转化情况。利用人工唾液、胃液和胰液、Caco - 2 单层细胞以及厌氧发酵模型在体外模拟口腔、胃、小肠和结肠的消化过程。结果表明，二 CQAs 在模拟唾液、胃液和胰液以及 Caco - 2 细胞内保持完整。在用人粪便悬液进行的厌氧发酵中，二 CQAs 被水解为单 CQAs 和咖啡酸，它们又分别进一步代谢为咖啡酸和二氢咖啡酸。二 CQAs 的水解取决于其化学结构、培养基中的碳水化合物以及微生物组成。我们的研究表明，二 CQAs 的水解发生在结肠中，由肠道微生物群介导。
