Xie Minhao, Chen Guijie, Wan Peng, Dai Zhuqing, Hu Bing, Chen Ligen, Ou Shiyi, Zeng Xiaoxiong, Sun Yi
College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, Jiangsu, China.
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology , Yancheng 224051, Jiangsu, China.
J Agric Food Chem. 2017 Nov 29;65(47):10185-10196. doi: 10.1021/acs.jafc.7b03992. Epub 2017 Nov 13.
Dietary polyphenols have been considered as novel prebiotics, and polyphenols could exert their functions through modulating intestinal microbiota. The diverse bioactivities of kudingcha could derive from its phenolic compounds, but the effects of dicaffeoylquinic acids (diCQAs) from Ilex kudingcha on intestinal microbiota have not been investigated. In the present study, high-throughput sequencing and anaerobic fermentation in vitro were utilized to investigate the microecology-modulating function of I. kudingcha diCQAs. As a result, diCQAs raised the diversity and exhibited a more considerable impact than a carbon source on the microbial profile. DiCQAs increased the relative abundances of Alistipes, Bacteroides, Bifidobacterium, Butyricimonas, Clostridium sensu stricto, Escherichia/Shigella, Parasutterella, Romboutsia, Oscillibacter, Veillonella, Phascolarctobacterium, Lachnospiracea incertae sedis, Gemmiger, Streptococcus, and Haemophilus and decreased the relative abundances of Ruminococcus, Anaerostipes, Dialister, Megasphaera, Megamonas, and Prevotella. DiCQAs also affected the generation of short-chain fatty acids through microbiota. The contents of acetic and lactic acids were raised, while the production of propionic and butyric acids was reduced. Conclusively, diCQAs from I. kudingcha had significant modulating effects on intestinal microbiota in vitro, which might be the fundamental of diCQAs exerting their bioactivities.
膳食多酚被认为是新型益生元，且多酚可通过调节肠道微生物群发挥其功能。苦丁茶的多种生物活性可能源于其酚类化合物，但苦丁茶中二咖啡酰奎宁酸（diCQAs）对肠道微生物群的影响尚未得到研究。在本研究中，利用高通量测序和体外厌氧发酵来研究苦丁茶diCQAs的微生态调节功能。结果显示，diCQAs提高了多样性，并且对微生物谱的影响比碳源更显著。diCQAs增加了阿利斯杆菌属、拟杆菌属、双歧杆菌属、丁酸单胞菌属、严格梭菌属、埃希氏菌属/志贺氏菌属、副萨特氏菌属、罗姆布茨菌属、颤杆菌属、韦荣球菌属、考拉杆菌属、不可培养的毛螺菌科、吉氏菌属、链球菌属和嗜血杆菌属的相对丰度，降低了瘤胃球菌属、厌氧棒状菌属、戴阿李斯特菌属、巨球形菌属、巨单胞菌属和普雷沃菌属的相对丰度。diCQAs还通过微生物群影响短链脂肪酸的生成。乙酸和乳酸含量升高，而丙酸和丁酸的生成减少。总之，苦丁茶中的diCQAs在体外对肠道微生物群具有显著的调节作用，这可能是diCQAs发挥其生物活性的基础。
