通用单克隆抗体的制备及用于检测动物饲料中吩噻嗪类药物的高灵敏度间接竞争酶联免疫吸附测定法的开发。
Preparation of a generic monoclonal antibody and development of a highly sensitive indirect competitive ELISA for the detection of phenothiazines in animal feed.
作者信息
Wang Juan, Wang Yulian, Pan Yuanhu, Chen Dongmei, Liu Zhenli, Feng Liang, Peng Dapeng, Yuan Zonghui
机构信息
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Wuhan, Hubei 430070, China; Huazhong Agricultural University, Wuhan, Hubei 430070, China.
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Wuhan, Hubei 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Wuhan, Hubei 430070, China; Huazhong Agricultural University, Wuhan, Hubei 430070, China.
出版信息
Food Chem. 2017 Apr 15;221:1004-1013. doi: 10.1016/j.foodchem.2016.11.062. Epub 2016 Nov 15.
In this study, a broadly specific monoclonal antibody was prepared and a sensitive monoclonal-based indirect competitive enzyme linked immunosorbent assay (ic-ELISA) was subsequently developed to determine the phenothiazines in animal feed with a simple sample preparation procedure for the first time. The obtained antibody 3A5 was of the immunoglobulin G1 (IgG1) isotype possessing a kappa light chain, which broadly cross-reacted to nine phenothiazines. The limit of detections of the method ranged from 1.1μgkg to 15.3μgkg in the swine feed and the fish feed. The recoveries of the phenothiazines were in the range of 78.2-116.6%. The coefficient of variations were less than 16.7%. A positive correlation (r>0.9249) between the results of the ic-ELISA and the high-performance liquid chromatography were also observed, which indicated that the developed ic-ELISA is reliable and can be used to monitor phenothiazines in animal feed.
在本研究中，制备了一种具有广泛特异性的单克隆抗体，随后开发了一种基于单克隆抗体的灵敏间接竞争酶联免疫吸附测定法（ic-ELISA），首次采用简单的样品制备程序来测定动物饲料中的吩噻嗪类药物。获得的抗体3A5属于具有κ轻链的免疫球蛋白G1（IgG1）同种型，它与九种吩噻嗪类药物具有广泛的交叉反应。该方法在猪饲料和鱼饲料中的检测限范围为1.1μg/kg至15.3μg/kg。吩噻嗪类药物的回收率在78.2%至116.6%之间。变异系数小于16.7%。还观察到ic-ELISA结果与高效液相色谱结果之间存在正相关（r>0.9249），这表明所开发的ic-ELISA是可靠的，可用于监测动物饲料中的吩噻嗪类药物。
Zotero 插件