广谱玉米赤霉烯酮及其主要类似物抗体的制备及其在间接竞争酶联免疫吸附测定中的应用。
Preparation of a broad-spectrum anti-zearalenone and its primary analogues antibody and its application in an indirect competitive enzyme-linked immunosorbent assay.
机构信息
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
出版信息
Food Chem. 2018 May 1;247:8-15. doi: 10.1016/j.foodchem.2017.12.016. Epub 2017 Dec 7.
A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for rapidly screening zearalenone (ZEN) and its primary analogues in various samples using an easy sample preparation procedure. Primarily, a group-specific mAb, 6C2, was produced, which had IC values for ZEN, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol and zearalanone of 114.0, 127.4, 290.4, 114.9, 205.6 and 257.1 ng L, respectively. The limit of detection and limit of quantitation of this method for ZEN and its five primary analogues in various matrix samples ranged from 114.2 to 812.3 ng L and 237.1 to 1653.9 ng L, respectively. The recoveries of the above samples spiked with ZEN and its five primary analogues were in the range of 62.9-113.6%. The CVs were less than 13.2%. A good correlation (R = 0.995) between the ic-ELISA results and the HPLC-MS/MS results for swine feeds supported the reliability of the developed ic-ELISA.
已开发出一种基于广谱单克隆抗体（mAb）的间接竞争酶联免疫吸附测定法（ic-ELISA），用于通过简单的样品制备程序快速筛选各种样品中的玉米赤霉烯酮（ZEN）及其主要类似物。首先，生产了一种组特异性 mAb，6C2，其对 ZEN、α-玉米赤霉烯醇、β-玉米赤霉烯醇、α-玉米赤醇、β-玉米赤醇和玉米赤霉烯酮的 IC 值分别为 114.0、127.4、290.4、114.9、205.6 和 257.1ng/L。该方法对各种基质样品中 ZEN 和其五种主要类似物的检测限和定量限范围为 114.2-812.3ng/L 和 237.1-1653.9ng/L。用 ZEN 和其五种主要类似物对上述样品进行加标回收，回收率在 62.9-113.6%之间。CV 小于 13.2%。猪饲料中 ic-ELISA 结果与 HPLC-MS/MS 结果之间具有良好的相关性（R=0.995），支持所开发的 ic-ELISA 的可靠性。
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