Martí Raúl, Valcárcel Mercedes, Herrero-Martínez José Manuel, Cebolla-Cornejo Jaime, Roselló Salvador
Unitat Mixta d'investigació en Millora de la Qualitat Agroalimentària UJI-UPV, Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain.
Departamento de Química Analítica, Universitat de València, Dr. Moliner, 50, 46100 Burjassot, Spain.
Food Chem. 2017 Apr 15;221:439-446. doi: 10.1016/j.foodchem.2016.10.105. Epub 2016 Oct 24.
A methodology for the determination of tomato phenolic acids and flavonoids has been developed combining MEKC and DAD detection. The influence on polyphenol separation of pH and background electrolyte, BGE (borax, acetonitrile, methanol and SDS concentrations), was studied and optimized using response surface methodology and weighted desirability function. Separation of polyphenols was achieved within 20min at 15°C using 11.3mM borax and 11.2mM SDS adjusted to pH 8.5 as BGE. Validation was performed using standards and tomato extracts. Recoveries ranged from 77 to 106%. Acceptable repeatabilities were obtained for peak area (%RSD <3.1% and <3.7%) and migration times (%RSD <0.2% and <1.4%) for intra- and inter-day respectively. Detection limits ranged between 0.8 and 3.8mgkg. Five and seven of these polyphenols were determined in samples of tomato and related species. This methodology will be valuable tool in breeding programs, analyzing a large number of samples.
已开发出一种结合毛细管电泳-质谱联用（MEKC）和二极管阵列检测（DAD）来测定番茄中酚酸和黄酮类化合物的方法。使用响应面法和加权期望函数研究并优化了pH值和背景电解质（硼砂、乙腈、甲醇和十二烷基硫酸钠浓度）对多酚分离的影响。在15℃下，使用11.3mM硼砂和11.2mM十二烷基硫酸钠（pH值调至8.5）作为背景电解质，20分钟内实现了多酚的分离。使用标准品和番茄提取物进行了验证。回收率在77%至106%之间。日内和日间峰面积（相对标准偏差<3.1%和<3.7%）和迁移时间（相对标准偏差<0.2%和<1.4%）的重复性均可接受。检测限在0.8至3.8mg/kg之间。在番茄及相关物种的样品中测定了其中的五种和七种多酚。这种方法将成为育种计划中分析大量样品的宝贵工具。
