Analytical Chemistry Department, Institute of Fine Chemistry and Nanochemistry, Campus of Rabanales, Annex to Marie Curie Building, University of Córdoba, 14071 Córdoba, Spain.
Food Chem. 2017 Apr 15;221:864-870. doi: 10.1016/j.foodchem.2016.11.085. Epub 2016 Nov 19.
A new type of magnetoliposomes (MLs), containing hydrophobic magnetic-gold nanoparticles and the long wavelength fluorophore cresyl violet, has been used for the determination of coenzyme Q10 (CoQ10). MLs were concentrated just before the detector, using a flow system and an external electromagnet device. The subsequent introduction of Triton X-100 and CoQ10 causes the MLs lysis and the cresyl violet oxidation, obtaining a decrease in the fluorescence signal. The dynamic range of the calibration graph was 0.03-0.50μmolL CoQ10, and the detection limit was 0.008μmolL. The precision (relative standard deviation) was in the range of 1.3-4.5%. The method showed a sampling frequency of 12h and was applied to the determination of CoQ10 in several food samples. The results were compared with those obtained using a previously described chromatographic method. Also, recovery values were in the range of 83.5-101.3%.
一种新型的磁脂体（MLs），含有疏水性磁性金纳米粒子和长波长荧光染料 Cresyl Violet，已被用于测定辅酶 Q10（CoQ10）。在检测器之前，使用流动系统和外部电磁装置浓缩 MLs。随后引入 Triton X-100 和 CoQ10 会导致 MLs 裂解和 Cresyl Violet 氧化，从而导致荧光信号减弱。校准曲线的动态范围为 0.03-0.50μmolL CoQ10，检测限为 0.008μmolL。精度（相对标准偏差）在 1.3-4.5%范围内。该方法的采样频率为 12 小时，已应用于几种食品样品中 CoQ10 的测定。结果与先前描述的色谱法得到的结果进行了比较。此外，回收率值在 83.5-101.3%范围内。
