• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

纳米通道电穿孔作为急性髓系白血病活细胞检测平台

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

作者信息

Zhao Xi, Huang Xiaomeng, Wang Xinmei, Wu Yun, Eisfeld Ann-Kathrin, Schwind Sebastian, Gallego-Perez Daniel, Boukany Pouyan E, Marcucci Guido I, Lee Ly James

机构信息

Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA; William G. Lowrie, Department of Chemical and Biomolecular Engineering The Ohio State University Columbus OH 43210 USA.

Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.

出版信息

Adv Sci (Weinh). 2015 Jul 16;2(12):1500111. doi: 10.1002/advs.201500111. eCollection 2015 Dec.

DOI:10.1002/advs.201500111
PMID:27980918
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5115302/
Abstract

is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells.

摘要

通过对单细胞中的RNA进行分析得以证明。这种微创过程基于单个细胞，通过顺序递送实现多靶点分析和刺激-反应分析。该独特平台在对稀有或难以转染的细胞进行全面且无裂解的核酸分析方面具有巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/7909cc8b7230/ADVS-2-0o-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/112c40171707/ADVS-2-0o-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/bcfcd7882a6e/ADVS-2-0o-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/7909cc8b7230/ADVS-2-0o-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/112c40171707/ADVS-2-0o-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/bcfcd7882a6e/ADVS-2-0o-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/5115302/7909cc8b7230/ADVS-2-0o-g003.jpg

相似文献

1
Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.纳米通道电穿孔作为急性髓系白血病活细胞检测平台
Adv Sci (Weinh). 2015 Jul 16;2(12):1500111. doi: 10.1002/advs.201500111. eCollection 2015 Dec.
2
Induced Apoptosis Investigation in Wild-type and FLT3-ITD Acute Myeloid Leukemia Cells by Nanochannel Electroporation and Single-cell qRT-PCR.通过纳米通道电穿孔和单细胞定量逆转录聚合酶链反应对野生型和FLT3-ITD急性髓系白血病细胞进行诱导凋亡研究。
Mol Ther. 2016 May;24(5):956-64. doi: 10.1038/mt.2016.6. Epub 2016 Jan 19.
3
3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.用于高通量细胞操作和电穿孔的3D纳米通道阵列
Methods Mol Biol. 2020;2050:29-41. doi: 10.1007/978-1-4939-9740-4_4.
4
Micromachining of Polymeric Microfluidic Micro/Nanoelectroporation Device.聚合物微流控微/纳米电穿孔装置的微加工
Methods Mol Biol. 2020;2050:21-27. doi: 10.1007/978-1-4939-9740-4_3.
5
3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control.用于高通量细胞转染的具有高均匀性和剂量控制的3D纳米通道电穿孔技术。
Nanoscale. 2016 Jan 7;8(1):243-52. doi: 10.1039/c5nr03187g.
6
Nanochannel electroporation delivers precise amounts of biomolecules into living cells.纳米通道电穿孔将精确数量的生物分子递送到活细胞中。
Nat Nanotechnol. 2011 Oct 16;6(11):747-54. doi: 10.1038/nnano.2011.164.
7
Nanochannel Electro-Injection as a Versatile Platform for Efficient RNA/DNA Programming on Dendritic Cells.纳米通道电注入作为一种用于树突状细胞中高效 RNA/DNA 编程的多功能平台。
Small. 2023 Oct;19(43):e2303088. doi: 10.1002/smll.202303088. Epub 2023 Jun 28.
8
Cellular Delivery of Large Functional Proteins and Protein-Nucleic Acid Constructs via Localized Electroporation.通过局部电穿孔实现大功能蛋白和蛋白-核酸构建体的细胞递送。
Nano Lett. 2023 Apr 26;23(8):3653-3660. doi: 10.1021/acs.nanolett.2c04374. Epub 2023 Feb 27.
9
A modified single-cell electroporation method for molecule delivery into a motile protist, Euglena gracilis.一种用于将分子导入运动性原生生物纤细裸藻的改良单细胞电穿孔方法。
J Microbiol Methods. 2016 Nov;130:106-111. doi: 10.1016/j.mimet.2016.08.018. Epub 2016 Aug 21.
10
Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform.在纳米通道阵列平台上对原代哺乳动物心肌细胞进行可控的大规模转染
Small. 2016 Nov;12(43):5971-5980. doi: 10.1002/smll.201601465. Epub 2016 Sep 20.

引用本文的文献

1
Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate.使用带有多孔基质的商业细胞培养插入物将基因电穿孔导入哺乳动物细胞。
Pharmaceutics. 2022 Sep 16;14(9):1959. doi: 10.3390/pharmaceutics14091959.
2
Recent Advances in Microscale Electroporation.微尺度电穿孔技术的最新进展
Chem Rev. 2022 Jul 13;122(13):11247-11286. doi: 10.1021/acs.chemrev.1c00677. Epub 2022 Jun 23.
3
Nanotechnology-Driven Cell-Based Therapies in Regenerative Medicine.纳米技术驱动的基于细胞的再生医学疗法。

本文引用的文献

1
Effect of nonendocytic uptake of nanoparticles on human bronchial epithelial cells.纳米颗粒的非内吞摄取对人支气管上皮细胞的影响。
Anal Chem. 2015 Mar 17;87(6):3208-15. doi: 10.1021/ac503366w. Epub 2015 Feb 26.
2
Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.使用纳米喷泉探针电穿孔分子信标对mRNA表达进行单细胞检测。
Small. 2015 May;11(20):2386-91. doi: 10.1002/smll.201401137. Epub 2015 Feb 1.
3
Indole-3-carbinol inhibits tumorigenicity of hepatocellular carcinoma cells via suppression of microRNA-21 and upregulation of phosphatase and tensin homolog.
AAPS J. 2022 Mar 15;24(2):43. doi: 10.1208/s12248-022-00692-3.
4
In Situ Deployment of Engineered Extracellular Vesicles into the Tumor Niche via Myeloid-Derived Suppressor Cells.通过髓系来源的抑制细胞在肿瘤微环境中原位递呈工程细胞外囊泡。
Adv Healthc Mater. 2022 Mar;11(5):e2101619. doi: 10.1002/adhm.202101619. Epub 2021 Oct 27.
5
Micromotor-based localized electroporation and gene transfection of mammalian cells.基于微电机的哺乳动物细胞局部电穿孔和基因转染。
Proc Natl Acad Sci U S A. 2021 Sep 21;118(38). doi: 10.1073/pnas.2106353118.
6
Early Intervention in Ischemic Tissue with Oxygen Nanocarriers Enables Successful Implementation of Restorative Cell Therapies.使用氧纳米载体对缺血组织进行早期干预可成功实施恢复性细胞疗法。
Cell Mol Bioeng. 2020 May 29;13(5):435-446. doi: 10.1007/s12195-020-00621-4. eCollection 2020 Oct.
7
Nanochannel-Based Poration Drives Benign and Effective Nonviral Gene Delivery to Peripheral Nerve Tissue.基于纳米通道的转孔作用可实现外周神经组织的良性、高效非病毒基因传递。
Adv Biosyst. 2020 Nov;4(11):e2000157. doi: 10.1002/adbi.202000157. Epub 2020 Sep 16.
8
Nanostructured Materials for Intracellular Cargo Delivery.用于细胞内货物递送的纳米结构材料。
Acc Chem Res. 2019 Sep 17;52(9):2462-2471. doi: 10.1021/acs.accounts.9b00272. Epub 2019 Aug 29.
9
A Review on Electroporation-Based Intracellular Delivery.基于电穿孔的细胞内递药研究综述。
Molecules. 2018 Nov 21;23(11):3044. doi: 10.3390/molecules23113044.
吲哚 - 3 - 甲醇通过抑制微小RNA - 21和上调磷酸酶及张力蛋白同源物来抑制肝癌细胞的致瘤性。
Biochim Biophys Acta. 2015 Jan;1853(1):244-53. doi: 10.1016/j.bbamcr.2014.10.017. Epub 2014 Oct 25.
4
Targeted delivery of tumor suppressor microRNA-1 by transferrin-conjugated lipopolyplex nanoparticles to patient-derived glioblastoma stem cells.通过转铁蛋白偶联的脂质多聚体纳米颗粒将肿瘤抑制性微小RNA-1靶向递送至患者来源的胶质母细胞瘤干细胞。
Curr Pharm Biotechnol. 2014;15(9):839-46. doi: 10.2174/1389201015666141031105234.
5
Microfluidic device for stem cell differentiation and localized electroporation of postmitotic neurons.用于干细胞分化和有丝分裂后神经元局部电穿孔的微流控装置。
Lab Chip. 2014 Dec 7;14(23):4486-95. doi: 10.1039/c4lc00721b. Epub 2014 Sep 10.
6
Insight into mechanisms of cellular uptake of lipid nanoparticles and intracellular release of small RNAs.深入了解脂质纳米颗粒的细胞摄取机制和小RNA的细胞内释放。
Pharm Res. 2014 Oct;31(10):2685-95. doi: 10.1007/s11095-014-1366-7. Epub 2014 Apr 17.
7
Intronic miR-3151 within BAALC drives leukemogenesis by deregulating the TP53 pathway.内含子 miR-3151 通过调节 TP53 通路驱动 BAALC 白血病发生。
Sci Signal. 2014 Apr 15;7(321):ra36. doi: 10.1126/scisignal.2004762.
8
Nonendocytic delivery of lipoplex nanoparticles into living cells using nanochannel electroporation.利用纳米通道电穿孔将脂质体纳米颗粒非内吞递送至活细胞。
Adv Healthc Mater. 2014 May;3(5):682-9. doi: 10.1002/adhm.201300213. Epub 2013 Aug 29.
9
Nanofountain probe electroporation (NFP-E) of single cells.纳米 Fountain 探针电穿孔（NFP-E）的单细胞。
Nano Lett. 2013 Jun 12;13(6):2448-57. doi: 10.1021/nl400423c.
10
Targeted delivery of microRNA-29b by transferrin-conjugated anionic lipopolyplex nanoparticles: a novel therapeutic strategy in acute myeloid leukemia.转铁蛋白修饰的阴离子脂多糖纳米载体靶向递送 miR-29b：急性髓系白血病的一种新治疗策略。
Clin Cancer Res. 2013 May 1;19(9):2355-67. doi: 10.1158/1078-0432.CCR-12-3191. Epub 2013 Mar 14.